Reporter: Week 12 August 1-6

From 2011.igem.org

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(Created page with "==Monday, August 1== J23100+RBS34 Results: no colony growth Colony PCR GFP+tev cleavage site GFP+cI cleavage site LacZα+small linker LacZα+Imp linker LacZ&alpha...")
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==Monday, August 1==
==Monday, August 1==
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J23100+RBS34
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Clone LacZα and GusA into K3
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Results: no colony growth
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Colony PCR
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GFP+tev cleavage site
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GFP+cI cleavage site
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LacZα+small linker
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LacZα+Imp linker
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LacZα+10AA linker
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XylE+10AA linker
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Results:GFP+cleavage sites failed, all else worked and were cultured
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==Tuesday, August 2==
==Tuesday, August 2==
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Colony PCR LacZα and GusA clones
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Results: failure
==Wednesday, August 3==
==Wednesday, August 3==
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Clone GusA and LacZα into K3
==Thursday, August 4==
==Thursday, August 4==
 +
Colony PCR LacZα and GusA clones
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Results: failure
==Friday, August 5==
==Friday, August 5==
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Place B0015 terminator on tail end of PR-RBS-XylE-small linker-tev cleavage site-GFP
==Saturday, August 6==
==Saturday, August 6==
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No colonies grew from August 5 assembly
[[Team:Penn_State/Lab| Back to Wetlab]]
[[Team:Penn_State/Lab| Back to Wetlab]]

Revision as of 07:13, 28 September 2011

Contents

Monday, August 1

Clone LacZα and GusA into K3

Tuesday, August 2

Colony PCR LacZα and GusA clones

Results: failure

Wednesday, August 3

Clone GusA and LacZα into K3

Thursday, August 4

Colony PCR LacZα and GusA clones

Results: failure

Friday, August 5

Place B0015 terminator on tail end of PR-RBS-XylE-small linker-tev cleavage site-GFP

Saturday, August 6

No colonies grew from August 5 assembly Back to Wetlab