Team:UC Davis/Project
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<h2>KO3D Plotting Library</h2> | <h2>KO3D Plotting Library</h2> | ||
When researching ways to present <a href="https://2011.igem.org/Team:UC_Davis/Data_LacI">LacI characterization data</a> clearly on this website, we realized there were no simple, cross-platform javascript libraries for interactive 3D data plotting. To rectify this, we coded our own. Read more about it <a href="https://2011.igem.org/Team:UC_Davis/KO3D">here</a>. | When researching ways to present <a href="https://2011.igem.org/Team:UC_Davis/Data_LacI">LacI characterization data</a> clearly on this website, we realized there were no simple, cross-platform javascript libraries for interactive 3D data plotting. To rectify this, we coded our own. Read more about it <a href="https://2011.igem.org/Team:UC_Davis/KO3D">here</a>. | ||
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Revision as of 06:26, 28 September 2011
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View our judging criteria for iGEM 2011 here.
Overview
We set out to develop a rugged process for the rapid production of mutant libraries of any BioBrick part using standard primers and a simple mutagenic PCR protocol. We chose to prototype this process by creating mutant libraries of the LacI, TetR and λ cI repressible promoters and to mutate GFP to visually assess our ability to create functional protein mutants.
As of October 2011, we have a functioning mutant library generation and screening process, and a selection of well-characterized promoter mutants, including seven LacI promoter mutants and seven λ cI promoter mutants. We also have nine TetR promoter mutants and eight GFP mutants (two of which have been lovingly named "Orange-Mutated Green Fluorescent Protein," or "OMGfp" 1 and 2) which await further characterization.
As of October 2011, we have a functioning mutant library generation and screening process, and a selection of well-characterized promoter mutants, including seven LacI promoter mutants and seven λ cI promoter mutants. We also have nine TetR promoter mutants and eight GFP mutants (two of which have been lovingly named "Orange-Mutated Green Fluorescent Protein," or "OMGfp" 1 and 2) which await further characterization.