Team:UC Davis/Project
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<h2>Project Selection</h2> | <h2>Project Selection</h2> | ||
- | Why would we want to make mutants? What's so special about repressible promoters? Read about how we came to decide on this project idea <a href="https://2011.igem.org/Team:UC_Davis/Project_Selection">here.</a | + | Why would we want to make mutants? What's so special about repressible promoters? Read about how we came to decide on this project idea <a href="https://2011.igem.org/Team:UC_Davis/Project_Selection">here.</a><br><br> |
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<h2>Process</h2> | <h2>Process</h2> | ||
- | Want to make your own mutant library? Curious as to how we assayed our promoter mutants, or how we selected variants that had well-spaced activity levels? Read about our library generation process <a href="https://2011.igem.org/Team:UC_Davis/Process">here</a>. | + | Want to make your own mutant library? Curious as to how we assayed our promoter mutants, or how we selected variants that had well-spaced activity levels? Read about our library generation process <a href="https://2011.igem.org/Team:UC_Davis/Process">here</a>. <br> |
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<h2>Promoter Mutants</h2> | <h2>Promoter Mutants</h2> | ||
- | We chose repressible promoters because they are useful in designing complex circuits and are relatively easy to screen for changes in activity level. You can view detailed information on our <a href="https://2011.igem.org/Team:UC_Davis/LacI">LacI</a>, <a href="https://2011.igem.org/Team:UC_Davis/TetR">TetR</a> and <a href="https://2011.igem.org/Team:UC_Davis/cilambda">λ cI</a> mutants on their respective pages. | + | We chose repressible promoters because they are useful in designing complex circuits and are relatively easy to screen for changes in activity level. You can view detailed information on our <a href="https://2011.igem.org/Team:UC_Davis/LacI">LacI</a>, <a href="https://2011.igem.org/Team:UC_Davis/TetR">TetR</a> and <a href="https://2011.igem.org/Team:UC_Davis/cilambda">λ cI</a> mutants on their respective pages.<br> |
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<h2>KO3D Plotting Library</h2> | <h2>KO3D Plotting Library</h2> | ||
- | When researching ways to present <a href="https://2011.igem.org/Team:UC_Davis/Data_LacI">LacI characterization data</a> clearly on this website, we realized there were no simple, cross-platform javascript libraries for interactive 3D data plotting. To rectify this, we coded our own. Read more about it <a href="https://2011.igem.org/Team:UC_Davis/KO3D">here</a>. | + | When researching ways to present <a href="https://2011.igem.org/Team:UC_Davis/Data_LacI">LacI characterization data</a> clearly on this website, we realized there were no simple, cross-platform javascript libraries for interactive 3D data plotting. To rectify this, we coded our own. Read more about it <a href="https://2011.igem.org/Team:UC_Davis/KO3D">here</a>. |
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Revision as of 06:26, 28 September 2011
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View our judging criteria for iGEM 2011 here.
Overview
We set out to develop a rugged process for the rapid production of mutant libraries of any BioBrick part using standard primers and a simple mutagenic PCR protocol. We chose to prototype this process by creating mutant libraries of the LacI, TetR and λ cI repressible promoters and to mutate GFP to visually assess our ability to create functional protein mutants.
As of October 2011, we have a functioning mutant library generation and screening process, and a selection of well-characterized promoter mutants, including seven LacI promoter mutants and seven λ cI promoter mutants. We also have nine TetR promoter mutants and eight GFP mutants (two of which have been lovingly named "Orange-Mutated Green Fluorescent Protein," or "OMGfp" 1 and 2) which await further characterization.
As of October 2011, we have a functioning mutant library generation and screening process, and a selection of well-characterized promoter mutants, including seven LacI promoter mutants and seven λ cI promoter mutants. We also have nine TetR promoter mutants and eight GFP mutants (two of which have been lovingly named "Orange-Mutated Green Fluorescent Protein," or "OMGfp" 1 and 2) which await further characterization.