Team:Panama/Protocols/Wetlab
From 2011.igem.org
(Difference between revisions)
Ernestopro (Talk | contribs) |
Ernestopro (Talk | contribs) |
||
Line 34: | Line 34: | ||
18. Cell Culture | 18. Cell Culture | ||
+ | |||
+ | [https://2011.igem.org/Team:Panama '''Home'''] |
Revision as of 05:43, 28 September 2011
1. Transformation
2. PCR
3. Colony PCR
4. Pgem Cloning
5. Preparing chemically competent cells
6. LB media recipie
7. Ampicilyn stock
8. Chloramfenicol Stock
9. Mutagenesis PCR
10. Mutagenesis transformation
11. Miniprep Quiagen Kit
12. Miniprep "Dirty"
13. PCR purification
14. Glicerol Stock
15. Agarose gel electrophorsis
16. Agar plates
17. Primer aliquots
18. Cell Culture