Team:Calgary/Notebook/Protocols/Process7
From 2011.igem.org
(Difference between revisions)
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</ol> | </ol> | ||
- | Equipment | + | <h4> Equipment </h4> |
+ | <ol> | ||
+ | |||
+ | <li>High-speed centrifuge and rotor</li> | ||
+ | <li>Spectrophotometer for chlorophyll measurements</li> | ||
+ | <li>Orbital shaker, light suitable for algal cell growth, and timer to control light settings</li> | ||
+ | <li>27-gauge * 0.5 inch stainless stell syringe needles</li> | ||
+ | <li>B-D Multifit 10mL glass Leuer-lock syringe</li> | ||
+ | <li>15mL Corex centrifuge tubes</li> | ||
+ | <li>Hemocytometer (cell counter)</li> | ||
+ | <li>Fine (artist style) paint brush</li> | ||
+ | </ol> | ||
</html> | </html> | ||
}} | }} |
Revision as of 01:43, 28 September 2011
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Plasmid Extraction
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This protocol will purify plasmids from bacteria, though identification of plasmids will need to be confirmed by PCR.
Reagents
- HEPES (N-[2-hydroxyethyl] piperazine N'[2-ethane-sulfonic acid])
- Sorbitol
- Percoll
- PEG 6000
- Ficoll
- Bovine serum albumin (BSA)
- Isoascorbic acid
- Sodium pyrophosphate
- Glutathione
- MgCl2.6H2O
- MnCl2
- Sodium-EDTA
Equipment
- High-speed centrifuge and rotor
- Spectrophotometer for chlorophyll measurements
- Orbital shaker, light suitable for algal cell growth, and timer to control light settings
- 27-gauge * 0.5 inch stainless stell syringe needles
- B-D Multifit 10mL glass Leuer-lock syringe
- 15mL Corex centrifuge tubes
- Hemocytometer (cell counter)
- Fine (artist style) paint brush
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