Team:Caltech/Week 2

From 2011.igem.org

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Setting up lab, stock supplies, preparation of LB-amp plates<br/>
Setting up lab, stock supplies, preparation of LB-amp plates<br/>
Test extraction of DNA from BioBrick wells into cloning plates<br/>
Test extraction of DNA from BioBrick wells into cloning plates<br/>
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Enrichment cultures of collected samples on minimal media with BPA</p>
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Initial enrichment cultures of collected samples on minimal media with BPA or 17a-ethynylestradiol</p>
== June 21 ==
== June 21 ==
<p>MoBio kit extraction of DNA from collected samples<br/>
<p>MoBio kit extraction of DNA from collected samples<br/>
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Enrichment cultures of samples in minimal media with 17α-estradiol, nonylphenol, and DDT<br/>
+
Initial enrichment cultures of samples in minimal media with nonylphenol or DDT<br/>
Geneious and primer design tutorial</p>
Geneious and primer design tutorial</p>
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==June 23==
==June 23==
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<p>PCR, digest, ligation and transformation of human estrogen receptor BioBrick to remove intron<br/>
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<p>Start cultures of RFP, transformed HER, T7 polymerase<br/>
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Primer design for constructs needed to test estrogen receptor<br/>
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Retest competent cells made June 22 with K123000 and pUC<br/>
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Transfer samples from enrichment cultures to new cultures<br/>
+
Transfer 0.5 mL aliquots of BPA and 17a-ethynylestradiol cultures from June 20 to new tubes of minimal media</p>
-
Start cultures of RFP, transformed HER, T7 polymerase</p>
+
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Revision as of 00:20, 24 June 2011


Caltech iGEM 2011



Home

Project

Data

Parts

Team

Notebook

Biosafety

Human Impact

References

Support

June 19

Collection of soil and water samples from LA River

June 20

Lab walkthrough
Setting up lab, stock supplies, preparation of LB-amp plates
Test extraction of DNA from BioBrick wells into cloning plates
Initial enrichment cultures of collected samples on minimal media with BPA or 17a-ethynylestradiol

June 21

MoBio kit extraction of DNA from collected samples
Initial enrichment cultures of samples in minimal media with nonylphenol or DDT
Geneious and primer design tutorial

June 22

Miniprep and sequencing of selected BioBricks (K123000, K123001, K123002, K123003)
Preparation of competent cells

June 23

Start cultures of RFP, transformed HER, T7 polymerase
Retest competent cells made June 22 with K123000 and pUC
Transfer 0.5 mL aliquots of BPA and 17a-ethynylestradiol cultures from June 20 to new tubes of minimal media


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