Team:UT Dallas/week5
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Revision as of 00:03, 28 September 2011
Week 5
8/8/11 |
Digestion: 1 hour and 30 minutes at 37 degrees Celsius |
8/9/11 |
Transformation of FGFR and CheZ* with pSB1C3 vector, 50 µL DH5a, 2 µL DNA, 37 degrees Celsius overnight, all chloramphenicol |
8/11/11 |
Incubate overnight in 3 mL LB chlora at 37 degrees Celsius and 220 rpm |
8/12/11 |
Post- incubation observation: |
8/13/11 |
Transformation (50 µL DH5a, 2 µL DNA, 37 degrees Celsius overnight, all carb) |
8/14/11 | Incubation in 3 mL LB carb overnight at 37 degrees Celsius and 220 rpm |