Team:UT Dallas/week6
From 2011.igem.org
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Test digestion for 1 hour and 30 minutes at 37 degrees Celsius<br> | Test digestion for 1 hour and 30 minutes at 37 degrees Celsius<br> | ||
Gel Electrophoresis<br> | Gel Electrophoresis<br> | ||
- | Test Digestion Observation: <br>Lane 5 gave positive results for FGF-R: 2 bands observed at<br> predicted bands size. Lane 4, 7, 9 were all negative bad results<br> because EcoRI was used but only 1 band showed. <br> This suggests that FGFR was not inserted.<br> Lanes 10, 11, 12 were CheZ* and all matched negative control. <br> Results are bad for CheZ*- will be regrown/miniprep/ligated.<br> | + | Test Digestion Observation: <br> Lane 5 gave positive results for FGF-R: 2 bands observed at<br> predicted bands size. Lane 4, 7, 9 were all negative bad results<br> because EcoRI was used but only 1 band showed. <br> This suggests that FGFR was not inserted.<br> Lanes 10, 11, 12 were CheZ* and all matched negative control. <br> Results are bad for CheZ*- will be regrown/miniprep/ligated.<br> |
Incubation in 3 mL LB chlora overnight at 37 degrees Celsius and 220 rpm | Incubation in 3 mL LB chlora overnight at 37 degrees Celsius and 220 rpm | ||
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Revision as of 23:52, 27 September 2011
Week 6
8/15/11 |
PCR of ptetR, RBS, LuxI, term |
8/16/11 |
Digestion; Gel Electrophoresis and Purification |
8/17/11 |
Glycerol stock ToxR and CheZ* colonies (2nd attempt) |
8/18/11 |
Glycerol Stock- 350 µL cells and 150 µL 50% glycerol |
8/19/11 | Transformation (DH5-alpha): Pcst-RBS-LuxI-Term (pSB1C3) -Chlora Glycerol Stock + Miniprep: pTet+mRFP, RBS-TetR-Term |
8/20/11 | Transformation: BBa_e0040 GFP Plate 1 Well 14K pSB1A2 Glycerol Stock/Miniprep: ctx-GFP |