Team:Calgary/Notebook/Protocols/Process20

From 2011.igem.org

(Difference between revisions)
Line 8: Line 8:
   <ul>
   <ul>
     <li> Overnight bacterial growth </li>
     <li> Overnight bacterial growth </li>
-
     <li> screw captubes </li>
+
     <li> screw cap tubes </li>
     <li> glycerol </li>
     <li> glycerol </li>
   </ul>
   </ul>
   <p> Protocol </p>
   <p> Protocol </p>
   <ol>
   <ol>
-
     <li> Pipet 0.5mL of 50% glycerol into 3 1.5 screw cap tubes</li>
+
     <li> Pipet 0.5mL of 50% glycerol into 3 1.5mL screw cap tubes</li>
-
     <li> Add 0.5mL of overnight culture to each tube </li>
+
     <li> Add 0.5mL of overnight culture to each tube. </li>
-
     <li> Pipet up and down to gently mix</li>
+
     <li> Pipet up and down to gently mix.</li>
-
     <li> Flash freeze  in liquid N2 or dry ice/ethanol bath </li>
+
     <li> Flash freeze  in liquid N2 or dry ice/ethanol bath. </li>
-
     <li> Place in -80 C freezer when frozen </li>
+
     <li> Place in -80°C freezer when frozen. </li>
   </ol>
   </ol>
</html>}}
</html>}}

Revision as of 22:49, 27 September 2011


Preparing Glycerol Stocks

Reagents and Materials

  • Overnight bacterial growth
  • screw cap tubes
  • glycerol

Protocol

  1. Pipet 0.5mL of 50% glycerol into 3 1.5mL screw cap tubes
  2. Add 0.5mL of overnight culture to each tube.
  3. Pipet up and down to gently mix.
  4. Flash freeze in liquid N2 or dry ice/ethanol bath.
  5. Place in -80°C freezer when frozen.

Retrieved from "http://2011.igem.org/Team:Calgary/Notebook/Protocols/Process20"