Team:Nevada/Project
From 2011.igem.org
Davidkoehn88 (Talk | contribs) (→Construct Components) |
Davidkoehn88 (Talk | contribs) (→Construct Components) |
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'''Kanamyacin resistance cassette (KanR):''' The kanamycin resistance cassette was PCR amplified from pUC4K (Taylor and Rose (1988) NAR 16:358). This cassette includes the neomycin phosphotransferase 2 gene with its own constitutive promoter and transcriptional terminator. This cassette is frequently used to select for positive transformants in Synechocystis. <br> | '''Kanamyacin resistance cassette (KanR):''' The kanamycin resistance cassette was PCR amplified from pUC4K (Taylor and Rose (1988) NAR 16:358). This cassette includes the neomycin phosphotransferase 2 gene with its own constitutive promoter and transcriptional terminator. This cassette is frequently used to select for positive transformants in Synechocystis. <br> | ||
- | '''Gibson Assembly of Components'''<br> | + | '''Gibson Assembly of Components:'''<br> |
'''Primer Design:''' Forward and reverse primers for each DNA part were designed with 20 base pair overlapping sequences with the upstream and downstream flanking segments. This will create 40 base pair overlaps between neighboring parts in the construct.<br> | '''Primer Design:''' Forward and reverse primers for each DNA part were designed with 20 base pair overlapping sequences with the upstream and downstream flanking segments. This will create 40 base pair overlaps between neighboring parts in the construct.<br> |
Revision as of 22:27, 27 September 2011
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