Team:UT Dallas/week8

From 2011.igem.org

(Difference between revisions)

Revision as of 22:18, 27 September 2011

Week 8

8/28/11	Transformation (DH5-alpha): GFP-RBS-TetR-term Incubation of CheZ, CheZ*

8/29/11	Glycerol/Miniprep ctx-CheZ, ctx-CheZ* Digestion: ToxR, FGFR Incubation ToxR

8/30/11	Test Digestion: ctx-CheZ, ctx-Chez, CheZ, CheZ Gel Electrophoresis: ctx-CheZ, ctx-Chez* Incubation: ToxR

8/31/11	Miniprep: ToxR Full Digestion: ToxR, pSB1C3 Gel Electrophoresis/Purifcation: ToxR,GFP -> CheZ, FGFR, RBS-TetR-Term Transformation: Ctx-CheZ, ToxR-FGFR, GFP-RBS-TetR-Term

9/1/11	Plating: k422007 CheY Incubation: GFP-RBS-TetR-Term (Carb) Incubation: E.Coli FHK12 - from Germany (Carb) Incubation: CheZ* (Chlora)

9/2/11	Plates Made: CarbR, ChloraR, CarbR/ChloraR Broth Made: LB CarbR, LB Carb/ChloraR Miniprep: GFP-RBS-TetR-Term, CheZ* Glycerol Stock: GFP-RBS-TetR-Term Made Competent Cells: FHK12 (Carb) Test Digestion/Gel Electrophoresis: GFP-RBS-TetR-Term, Full Digestion: CheZ, FGFR Ful Digestion: GFP-RBS-TetR-Term Transformation: German Parts=[pHKToxR'MalE, pHKToxR'MalE/ZIP, pHKToxR'REI-T39K, pHKToxR Plasmid stock: Diluted German parts (as above) Ligation 10 Min: ctx, ToxR into CheZ, FGFR respectively Transformation: CheZ, ctx-CheZ, FGFR, ToxR-FGFR Ligation Overnight: Vectors= GFP-RBS-TetR-Term, PyeaR, scp Inserts= ctx, GFP-RBS, TetR-Term

9/3/11	Transformation of the followings: ctx-GFP-RBS-TetR-Term, PyeaR-GFP-RBS-TetR-Term, scp-GFP-RBS-TetR-Term

@@ Line 2: / Line 2: @@
 <table>
 <tr><td valign='top'>8/28/11</td><td valign='top'>Transformation (DH5-alpha): GFP-RBS-TetR-term<br>
-Incubation of CheZ, CheZ*</td>
+Incubation of CheZ, CheZ*</td></tr>
 <tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
 <tr><td valign='top'>8/29/11</td><td valign='top'> Glycerol/Miniprep ctx-CheZ, ctx-CheZ*
 Digestion: ToxR, FGFR
-Incubation ToxR</td>
+Incubation ToxR</td></tr>
 <tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
 <tr><td valign='top'>8/30/11</td><td valign='top'> Test Digestion: ctx-CheZ, ctx-Chez*, CheZ, CheZ*<br>
 Gel Electrophoresis: ctx-CheZ, ctx-Chez*<br>
-Incubation: ToxR</td>
+Incubation: ToxR</td></tr>
 <tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
 <tr><td valign='top'>8/31/11</td><td valign='top'> Miniprep: ToxR<br>
 Full Digestion: ToxR, pSB1C3<br>
 Gel Electrophoresis/Purifcation: ToxR,GFP -> CheZ*, FGFR, RBS-TetR-Term<br>
-Transformation: Ctx-CheZ*, ToxR-FGFR, GFP-RBS-TetR-Term</td>
+Transformation: Ctx-CheZ*, ToxR-FGFR, GFP-RBS-TetR-Term</td></tr>
 <tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
 <tr><td valign='top'>9/1/11</td><td valign='top'> Plating: k422007 CheY<br>
 Incubation: GFP-RBS-TetR-Term (Carb)<br>
 Incubation: E.Coli FHK12 - from Germany (Carb)<br>
-Incubation: CheZ* (Chlora)</td>
+Incubation: CheZ* (Chlora)</td></tr>
 <tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
 <tr><td valign='top'>9/2/11</td><td valign='top'> Plates Made: CarbR, ChloraR, CarbR/ChloraR<br>
@@ Line 35: / Line 35: @@
 Transformation: CheZ*, ctx-CheZ*, FGFR, ToxR-FGFR<br>
 Ligation Overnight: Vectors= GFP-RBS-TetR-Term, PyeaR, scp  <br>
-		 Inserts= ctx, GFP-RBS, TetR-Term</td>
+		 Inserts= ctx, GFP-RBS, TetR-Term</td></tr>
 <tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
 <tr><td valign='top'>9/3/11</td><td valign='top'> Transformation of the followings:<br>ctx-GFP-RBS-TetR-Term, PyeaR-GFP-RBS-TetR-Term, scp-GFP-RBS-TetR-Term</td>
 </table>