Team:Calgary/Notebook/Protocols
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process3">cDNA Synthesis</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process3">cDNA Synthesis</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process4">Quantitative Real Time PCR</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process4">Quantitative Real Time PCR</a></li> | ||
- | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/pBAD"> | + | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/pBAD">Testing the pBAD Construct</a></li> |
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process5">Preparation of Algal f2 Media</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process5">Preparation of Algal f2 Media</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process6">Glass Bead Algal transformation</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process6">Glass Bead Algal transformation</a></li> | ||
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process10">PCR Purification</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process10">PCR Purification</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process11">Gel Extraction</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process11">Gel Extraction</a></li> | ||
- | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process12"> | + | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process12">Testing the LacI-LacZ I732901 gene</a></li> |
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process13">Bacterial Transformation</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process13">Bacterial Transformation</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process14">Rehydration of Registry DNA</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process14">Rehydration of Registry DNA</a></li> |
Revision as of 21:52, 27 September 2011
Protocols
Hello! Our wiki is currently under heavy construction, so please check regularly for updates. Thank you for your patience.
Here is a list of the procedures we used this summer. Each one has a description and list of materials needed.
- Plasmid Extraction
- RNA Extraction
- cDNA Synthesis
- Quantitative Real Time PCR
- Testing the pBAD Construct
- Preparation of Algal f2 Media
- Glass Bead Algal transformation
- Chloroplast Isolation
- Nuclear DNA extraction from Algae
- Taq PCR Protocol
- PCR Purification
- Gel Extraction
- Testing the LacI-LacZ I732901 gene
- Bacterial Transformation
- Rehydration of Registry DNA
- Restriction Digest