Team:UT Dallas/week7

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Revision as of 21:41, 27 September 2011

Week 7

8/21/11Incubation: GFP
  
8/22/11Glycerol Stock/Miniprep GFP

Test Digestion + Gel Electrophoresis: GFP
Dissolving/Diluting Primers for ctx promoter and FGFR

PCR/Gel purification: ctx
  
8/23/11Full Digestion: CheZ, CheZ*, ctx promoter

Dissolving/Diluting Primers: -----------------------------
Gel Electrophoresis/ Purification of CheZ, CheZ*

Ligation: ctx with CheZ, CheZ*
  
8/24/11PCR Point Mutation: FGFR (get rid of EcoRI restriction site)

Transformation (XL10 ultra competent): FGFR (point mutated)

Transformation: Ctx-CheZ (Carb), ctx-CheZ* (Chlora)
  
8/25/11Incubation: FGFR

Digestion, Gel Electrophoresis/Purification: CheZ, CheZ*

Ligation Overnight: Ctx --> CheZ, CheZ*
  
8/26/11Glycerol Stock/Miniprep: FGFR

Transformation CheZ, CheZ*
Test Digestion, Gel Electrophoresis

Made LbB Chlora & LB Kan
  
8/27/11Digestion, Gel Electrophoresis/Purification: RBS-TetR, GFP

Ligation (10 min): GFP into RBS-TetR-double term

Transformation: GFP-RBS-TetR-term
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