Team:Calgary/Notebook/Protocols/Process3

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Revision as of 17:01, 27 September 2011

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  • cDNA Synthesis by Reverse Transcription

    Component Volume
    RNA (40ng – 10ng to 1μg total RNA/rxn) variable
    Nuclease free water variable
    qScript cDNA synthesis mix 4 uL
    Final Volume 20 uL