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Team:Calgary/Notebook/Protocols/Process3
From 2011.igem.org
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<tr> | <tr> | ||
| - | <td width="85%"><div class="heading"><p style="font-size:14px; font-weight:bold"><a style="text-decoration:none" name=" | + | <td width="85%"><div class="heading"><p style="font-size:14px; font-weight:bold"><a style="text-decoration:none" name="RT"></a>cDNA Synthesis by Reverse Transcription </p></div></td> |
</tr> | </tr> | ||
</table> | </table> | ||
<table width="630" border="1px" style="margin-bottom:15px;"> | <table width="630" border="1px" style="margin-bottom:15px;"> | ||
<tr> | <tr> | ||
| - | <td><b> | + | <td><b>Component</b></td> |
| - | <td><b>Volume | + | <td><b>Volume</b></td> |
| - | + | ||
| - | + | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>RNA (40ng – 10ng to 1μg total RNA/rxn)</td> |
| - | + | <td>variable</td> | |
| - | + | ||
| - | + | ||
| - | <td> | + | |
</tr> | </tr> | ||
| + | <tr> | ||
| + | <td>Nuclease free water</td> | ||
| + | <td>variable</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>qScript cDNA synthesis mix</td> | ||
| + | <td>4 uL</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Final Volume</td> | ||
| + | <td>20 uL</td> | ||
| + | </tr> | ||
| + | </table> | ||
Revision as of 17:00, 27 September 2011
TITLE=cDNA Synthesis/Reverse Transcription Reaction| BODY=
