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Team:Brown-Stanford/Lab/Protocols/DM3 plates
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(Created page with "== '''DM3 Plates''' == DM3 plates: *Agar 200ml 4% *Sodium Succinate 500ml 1M pH 7.3 *Casamino Acids 100ml 5% *Yeast Extract 50ml 10% *K2HPO4 3.5% + KH...")
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(Created page with "== '''DM3 Plates''' == DM3 plates: *Agar 200ml 4% *Sodium Succinate 500ml 1M pH 7.3 *Casamino Acids 100ml 5% *Yeast Extract 50ml 10% *K2HPO4 3.5% + KH...")
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Revision as of 07:20, 27 September 2011
DM3 Plates
DM3 plates:
- Agar 200ml 4%
- Sodium Succinate 500ml 1M pH 7.3
- Casamino Acids 100ml 5%
- Yeast Extract 50ml 10%
- K2HPO4 3.5% + KH2PO4 1.5% in 100ml
- Glucose 15ml 20%
- MgCl2 20ml 1M
- BSA 5ml 2%
Notes
- Filter sterilize BSA and add when mixture is ~55˚C
