Team:Panama/Notebook/June2011

From 2011.igem.org

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We did a lot of work today like:  
We did a lot of work today like:  
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1. The miniprep of the strains containing the BioBrick rh1AB.  
1. The miniprep of the strains containing the BioBrick rh1AB.  
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2. An electrophoresis in agarose gel at 1.5% in wich we observed the plasmidic DNA.  
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2. An electrophoresis in agarose gel at 1.5% in wich we observed the plasmidic DNA.
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3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium.
3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium.
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4. Last attempt in seed the E. coli containing the rh1AB mutated from 2/3/11 in LB medium but it didn´t grow again! =(. This time we putted the exact concentration of cloranfenicol  (33 ug/ml).  
4. Last attempt in seed the E. coli containing the rh1AB mutated from 2/3/11 in LB medium but it didn´t grow again! =(. This time we putted the exact concentration of cloranfenicol  (33 ug/ml).  
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5. We did all the necessary calculations for the antibiotics concentrations.
 
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5. We did all the necessary calculations for the antibiotics concentrations.                                           
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                                  '''Rh1AB + psB1C3 Ectroforesis'''
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                                      [[File:e29-7-11.jpg]]
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== '''Rh1AB + psB1C3 Ectroforesis''' ==
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  [[File:e29-6-11.jpg]]                  
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== 1 and 8: Marker 100- 3000 pb. 2-7: rh1AB 2010 BioBrick ==

Revision as of 16:47, 26 September 2011