Team:Colombia/Notebook

From 2011.igem.org

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: Confirm all biobricks (Digestion).
: Confirm all biobricks (Digestion).
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===July 19===
 +
* Pass strain of Vibrio fischeri to blood agar base.
 +
* Check the growth of the isolates
 +
* Pass the transformed bricks 6, 7, 9 and 10.
 +
* Pass the isolates the solid media to liquid media (2,4,5 and 8)
 +
===July 21===
 +
July 21, 2011
 +
    Digestion to confirm No. 1, 3 and 4
-
TO BE CONTINUED...
+
{| border="1" align="center" style="text-align:center;"
-
 
+
|1X           
 +
|6X
 +
|H2O
 +
|29,4µL
 +
|160,4 µL
 +
|Buffer N. 3
 +
|4 µL
 +
|24 µL
 +
|EcoRI
 +
|0,3 µL
 +
|1,8 µL
 +
|PstI
 +
|0,3 µL
 +
|1,8 µL
 +
|DNA
 +
|7 µL
 +
|----
 +
|40 µL
 +
|}
=='''August'''==
=='''August'''==

Revision as of 15:28, 26 September 2011

Template:Https://2011.igem.org/User:Tabima

Contents

iGemColombia Notebook

Here you can find our daily work in the Lab!

June

June 30:

  • Biobricks 1, 2, 3, 4 and 5 were resuspended
  • Miniprep Solutions (I, II and III) were prepared.

July

July 1

  • Biobricks 1, 2, 3, 4 and 5 were resuspended.

July 5

  • Biobrick 2 presented no colonies.
  • Colonies from bricks 1, 3, 4 and 5 were stinged.
Task:
To make LB medium (15x25mL)

July 6

  • Biobricks 1, 3 and 4 were twice plated.
  • Brick 5 didn't grow up.
  • We've added Ampiciline (3.75mL) and Kanamicine (1.875mL) to the boxed from the previous day.
  • LB medium was prepared (15x25mL).
  • Liquid LB was prepared (400mL).
Task:
To add ampiciline and tetracicline to the boxes.
Claim the liquid LB
Scrape ....
Sting biobricks 2 and 5 (no clons)
Pick up the tubes with Merceditas

July 7

  • Clons 2 and 5 didn't work out.
  • Clons 1, 3 and 4 were planted in LB liquid.
  • E. Coli inoculation in Coffee
  • Kanamicine resistence plasmid: 0.2 optic density.
  • Direct inoculation x 2 and C(-) MgCl2.
Task:
Print electroporation protocol.
Ask Juan D. Olarte about the inoculation in coffee.
Minipreps for confirmation of 1, 3 and 4.
Competent cells for clons 2 and 5.

July 8

  • Minipreps for 1, 3 and 4 were made.
  • Plant sheets of coffee into different plates: Each sheet was cut in the middle and they were incubated at 25°C and 37°C LB+Kan.
Task:
To finish the minipreps from the addition of RNAsa.
Check the E. Coli growth in coffee.

July 11

  • Minipreps have been finished. They've been planted in gel: Low concentration (25 ng/uL).
  • Transformation protocol in chemical cells.
Task:
Prepare 250 mL of SOC medium
Print the Transformation protocol for chemical cells.

July 12

  • Strains 1 and 4 have been conserved.
  • LB liquid culture was prepared again for brick 3.
  • E. Coli growth results.
Task:
Print the Transformation protocol for chemical cells.
Competent cells for clons 2 and 5.
Preserve brick 3.
Confirm bricks 1, 3 and 4 (Digestion)
Resuspend all Biobricks.
Check the E. Coli growth in coffee.

July 14

  • E. Coli didn't grow up on the sheets.
  • Chemical competent cells were made (DH5α).
  • Brick 3 was left to grow in liquid medium.
  • Bricks 2, 5, 6,7, 8, 9 and 10 were transformed and plated.
Task:
Print the Transformation protocol for chemical cells.
Preserve brick 3.
Confirm all biobricks (Digestion)
Sting the transformed bricks.
Add antibiotic to the mediums made today.

July 15

  • Brick 3 was preserved in Revco.
  • Bricks 2, 5 and 8 were stinged.
  • 25 LB+Kan boxes x 25 mL.
  • No colonies in brick 6.
  • Bricks 7, 9 and 10 were contaminated.
Task:
Print the chemical cells protocol.
Confirm all biobricks (Digestion).

July 19

  • Pass strain of Vibrio fischeri to blood agar base.
  • Check the growth of the isolates
  • Pass the transformed bricks 6, 7, 9 and 10.
  • Pass the isolates the solid media to liquid media (2,4,5 and 8)

July 21

July 21, 2011 

   Digestion to confirm No. 1, 3 and 4
1X 6X H2O 29,4µL 160,4 µL Buffer N. 3 4 µL 24 µL EcoRI 0,3 µL 1,8 µL PstI 0,3 µL 1,8 µL DNA 7 µL
40 µL

August

Retrieved from "http://2011.igem.org/Team:Colombia/Notebook"