From 2011.igem.org
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| - | After we read an article about how yeast copes with toxic metals in its environment (Wysocki & Tamas, 2010), we made our preliminary decision of engineering a water tester. We discussed what metals we wanted to detect in the environment, and we decided on copper, becase yeast already has a promoter associated with copper, Cup1. We also decided to engineer the yeast cells to fluoresce red, and we chose a translational unit from the iGem registry that would work in yeast. It uses the RFP part to make the yeast fluoresce red. We researched fluorescence, and actually learned the concept behind it. Fluorescence is when waves of light with a high energy level hit the electrons of a substance of a low energy level. The high evergy level waves "excite" the electrons, and moves them into a higher energy orbital for a short time. When the electrons move back down to their original energy state, they have to release energy. This energy is released in the form of light. The color depends on the wavelength of the light, which in turn depends on the difference in the excited energy level of the electron and the normal energy state of the electron. To fluoresce red, the wavelengths emitted must be around 700 wavelengths long.
| + | Discussion of Gibson Assembly and if we should use Gibson as our standard assembly procedure. |
| | + | Group decided to use Gibson. |
| | + | Investigated the primers and the genes in yeast to use for the metal sensor |
| | + | Developed primers for the extraction from yeast and Gibson |
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| | <html><a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/Notebook"><b>Return to Notebook</b></a></html> | | <html><a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/Notebook"><b>Return to Notebook</b></a></html> |
Latest revision as of 04:30, 23 June 2011
April 20th:
Discussion of Gibson Assembly and if we should use Gibson as our standard assembly procedure.
Group decided to use Gibson.
Investigated the primers and the genes in yeast to use for the metal sensor
Developed primers for the extraction from yeast and Gibson
Return to Notebook
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