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Sensor: Week 5 June 12-17
From 2011.igem.org
(Difference between revisions)
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| - | ==Sunday== | + | ==Sunday 12 June 2011== |
===cI Repressor Test Assembly, Day 18=== | ===cI Repressor Test Assembly, Day 18=== | ||
| - | The Sensor group ran PCR of the K648000+K648001 using taq polymerase. | + | The Sensor group ran PCR of the K648000+K648001 using taq polymerase. The PCR products will be inserted into the O<sub>R</sub> vector. |
| - | ==Monday== | + | ==Monday 13 June 2011== |
===cI Repressor Test Assembly, Day 19=== | ===cI Repressor Test Assembly, Day 19=== | ||
| - | The assemblies that were cultured on 6/10/11 (J23113+B0030+C0051, J23113+B0031+C0051, B0032+C0051 were sent to sequencing. The results showed that only the vector grew, meaning that '''the assemblies will have to be performed.''' | + | The assemblies that were cultured on 6/10/11 (J23113+B0030+C0051, J23113+B0031+C0051, B0032+C0051 were sent to sequencing. The results showed that only the vector grew, meaning that '''the assemblies will have to be performed.''' |
| + | |||
| + | The O<sub>R</sub> site was placed in front of the cI repressor testing construct assembled on Monday, 6/6/11 as well as in front of a terminator and mCherry reporter. | ||
| + | |||
| + | {|border="1" | ||
| + | !Assembly Step | ||
| + | |align="center" colspan="2"| '''Part Involved with Assembly Step''' | ||
| + | |- | ||
| + | |rowspan="2"|Restriction Digest | ||
| + | |Insert Using SpeI and PstI: | ||
| + | |O<sub>R</sub> | ||
| + | |- | ||
| + | |Vectors Using XbaI and PstI: | ||
| + | |K648000+K648001<br />B0034+mCherry | ||
| + | |- | ||
| + | |Ligation | ||
| + | |colspan="2"|OR + K648000+K648001<br />OR + B0034+mCherry | ||
| + | |- | ||
| + | |Transformation/Plating | ||
| + | |colspan="2"|The above ligations were transformed into Escherichia coli cells and plated onto | ||
| + | |||
| + | |||
Revision as of 20:16, 22 June 2011
Contents |
Sunday 12 June 2011
cI Repressor Test Assembly, Day 18
The Sensor group ran PCR of the K648000+K648001 using taq polymerase. The PCR products will be inserted into the OR vector.
Monday 13 June 2011
cI Repressor Test Assembly, Day 19
The assemblies that were cultured on 6/10/11 (J23113+B0030+C0051, J23113+B0031+C0051, B0032+C0051 were sent to sequencing. The results showed that only the vector grew, meaning that the assemblies will have to be performed.
The OR site was placed in front of the cI repressor testing construct assembled on Monday, 6/6/11 as well as in front of a terminator and mCherry reporter.
| Assembly Step | Part Involved with Assembly Step | |
|---|---|---|
| Restriction Digest | Insert Using SpeI and PstI: | OR |
| Vectors Using XbaI and PstI: | K648000+K648001 B0034+mCherry | |
| Ligation | OR + K648000+K648001 OR + B0034+mCherry | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto
|
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