Team:WashU/Notebook/Transformation

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Revision as of 21:14, 25 September 2011

Transformation Group

The goal of the transformation team was to successful integrate the four synthesized genes into the yeast genome.

Before the genes were ready, we practiced by transformation by inserting extra DNA into the genome.

When the genes are ligated with the selection markers, we will use the added homology to integrate the linear piece of DNA into the yeast genome. After inserting one gene per yeast cell, we will mate the yeast twice and sporulate in order to isolate the yeast cells that have all four genes present.

Additional, this team transformed plasmids into e. coli in order to amplify plasmids for use by the other teams. This procedure consisted of transforming the plasmid into the bacterial genome, culturing the transformed bacteria, and isolating the DNA plasmid with a miniprep kit.

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 == Transformation Group ==
-The transformation team worked on the bacterial transformation. The team took any DNA plasmid and transforms the plasmid into the bacterial genome. We then cultured the transformed bacteria and isolate the DNA plasmid. This procedure amplifies the amount of DNA plasmid.
-The team also transformed DNA plasmid into yeast genome. After the microbiology group ligate the genes in the plasmid, we were responsible for inserting the genes into yeast.  We were responsible for sporulation of the yeast with different gene insertions to produce daughter cells with both genes of interest.
+The goal of the transformation team was to successful integrate the four synthesized genes into the yeast genome.
+Before the genes were ready, we practiced by transformation by inserting extra DNA into the genome.
+When the genes are ligated with the selection markers, we will use the added homology to integrate the linear piece of DNA into the yeast genome. After inserting one gene per yeast cell, we will mate the yeast twice and sporulate in order to isolate the yeast cells that have all four genes present.
+Additional, this team transformed plasmids into e. coli in order to amplify plasmids for use by the other teams. This procedure consisted of transforming the plasmid into the bacterial genome, culturing the transformed bacteria, and isolating the DNA plasmid with a miniprep kit.