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Team:Kyoto/Hunger
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= '''Project Hunger''' = | = '''Project Hunger''' = | ||
| - | Production of needless enzymes is a heavy burden especially when the resource is scarce. This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC<html><a href="#dagger1"><sup>†</sup></a></html> which activate σ<sup>54</sup>promoter when the supply of nitrogen is not enough. NtrB and NtrC are coded in | + | Production of needless enzymes is a heavy burden especially when the resource is scarce. This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC<html><a href="#dagger1"><sup>†</sup></a></html> which activate σ<sup>54</sup>promoter when the supply of nitrogen is not enough. NtrB and NtrC are coded in glnL and glnG, respectively. Here, we evaluated the relationships between expression under σ<sup>54</sup>promoter and that of these genes by the aid of RPU(a relative promoter unit). |
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Glutamate + NH<sub>3</sub> + ADP → Glutamine + ADP + phosphate | Glutamate + NH<sub>3</sub> + ADP → Glutamine + ADP + phosphate | ||
GS | GS | ||
| - | The expression of glnA is regulated by several proteins including NtrB, NtrC, Pii. | + | The expression of glnA is regulated by several proteins including NtrB, NtrC, Pii. |
| - | + | Fig. NtrCがσ54,RNAポリメラーゼ,DNAの関係図(窒素源あり/なし) | |
| - | + | Fig. NtrBCの関係図 | |
| + | Fig. Ntr, Pii, GSの関係図 | ||
== '''Method''' == | == '''Method''' == | ||
== '''Result''' == | == '''Result''' == | ||
== '''Discussion''' == | == '''Discussion''' == | ||
| + | == '''Reference''' == | ||
</div> | </div> | ||
Revision as of 14:59, 25 September 2011
Contents |
Project Hunger
Production of needless enzymes is a heavy burden especially when the resource is scarce. This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC† which activate σ54promoter when the supply of nitrogen is not enough. NtrB and NtrC are coded in glnL and glnG, respectively. Here, we evaluated the relationships between expression under σ54promoter and that of these genes by the aid of RPU(a relative promoter unit).
† NtrB and NtrC are otherwise called NRII, NRI
ところでこの遺伝子はもともとBioBrickに登録されていたものか、今回新たに作ったもののどちらなのでしょう
http://partsregistry.org/wiki/index.php/Part:BBa_J64978
などがもう登録されているようですが
Introduction
For every living thing, needless biological activity is not efficient. Cells must be controlled so that enzymes are produced only when they are necessary. Ammonia is an essential nitrogen source for the bactria. When enteric bacteria are deprived of ammonia, they express glnA to produce glutamine synthetase(GS). Nitrogen is used in the reaction of
Glutamate + NH3 + ADP → Glutamine + ADP + phosphate
GS
The expression of glnA is regulated by several proteins including NtrB, NtrC, Pii.
Fig. NtrCがσ54,RNAポリメラーゼ,DNAの関係図(窒素源あり/なし) Fig. NtrBCの関係図 Fig. Ntr, Pii, GSの関係図
