Team:KIT-Kyoto/yokoigawa

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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">8/29(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">月</span><span lang="EN-US">)</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">武田、横井川</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【目的】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物と</span><span lang="EN-US">pUAST-flag vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">の制限酵素処理（一回目：</span><span lang="EN-US">Xho 1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【実験操作】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">～フェノールクロロホルム処理・エタノール沈殿～</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">DAIP2</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">の</span><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物を</span><span lang="EN-US">400</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">まで</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">で希釈し、フェノール・クロロホルムを</span><span lang="EN-US">400</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">加えて</span><span lang="EN-US">voltex </span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し</span><span lang="EN-US">10000rpm3min4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓水層のみを新しいチューブに移した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓クロロホルムを等量加えて、</span><span lang="EN-US">voltex</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し</span><span lang="EN-US">10000rpm3min4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓水層のみを新しいチューブに移し</span><span lang="EN-US">3M CH<sub>3</sub>COONa(PH 5.27)</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">1/10</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">等量加え、</span><span lang="EN-US">isopropanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を等量加えた。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">3</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">分静置後、</span><span lang="EN-US">14000rpm 10min 4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓遠心後、上清を捨て、</span><span lang="EN-US">70</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">％</span><span lang="EN-US">ethanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">200</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">加え、</span><span lang="EN-US">15000rpm 5min 4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓遠心後、上清を捨て乾燥させる。乾燥後</span><span lang="EN-US">milliQ44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を加えて</span><span lang="EN-US">voltex</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し、</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">中に</span><span lang="EN-US">DNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を溶解させる。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">～制限酵素処理～</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">フェノールクロロホルム処理後下記の反応液を調整した</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">反応液調整（</span><span lang="EN-US">cDNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物</span><span lang="EN-US"> in milliQ<span style="mso-spacerun: yes">&nbsp;&nbsp;
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</span>44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>10x H Buffer</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">　　　　</span><span lang="EN-US">5</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>Xho 1<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp; </span>1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><?xml:namespace prefix ="" o ns
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="" "urn:schemas-microsoft-com:office:office" /><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">　　反応液調整（</span><span lang="EN-US">vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>pUAST-flag
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vector(500ng/</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l)<span style="mso-spacerun: yes">&nbsp; </span>10</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>milliQ<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp; </span>34</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>10x H
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Buffer<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp;&nbsp;&nbsp; </span>5</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>Xho 1<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓反応液調整後、</span><span lang="EN-US">37</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で</span><span lang="EN-US">20</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">時間静置</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p><span style="FONT-FAMILY: 'Century','serif'; FONT-SIZE: 10.5pt; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 11.0pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: EN-US; mso-fareast-language: JA; mso-bidi-language: AR-SA" lang="EN-US"><br style="PAGE-BREAK-BEFORE: always; mso-special-character: line-break" clear="all"></span>&nbsp;</p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt; mso-pagination: widow-orphan" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">8/30(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">火</span><span lang="EN-US">)</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">武田、横井川</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【目的】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物と</span><span lang="EN-US">pUAST-flag vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">の制限酵素処理</span><span lang="EN-US">(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">二回目：</span><span lang="EN-US">Xbal)</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【実験操作】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">制限酵素処理</span><span lang="EN-US">20h</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">後、フェノールクロロホルム処理を行う</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物を</span><span lang="EN-US">400</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">まで</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">で希釈し、フェノール・クロロホルムを</span><span lang="EN-US">400</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">加えて</span><span lang="EN-US">voltex </span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し</span><span lang="EN-US">10000rpm3min4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓水層のみを新しいチューブに移した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓クロロホルムを等量加えて、</span><span lang="EN-US">voltex</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し</span><span lang="EN-US">10000rpm3min4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓水層のみを新しいチューブに移し</span><span lang="EN-US">3M CH<sub>3</sub>COONa(PH 5.27)</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">1/10</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">等量加え、</span><span lang="EN-US">isopropanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を等量加えた。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">3</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">分静置後、</span><span lang="EN-US">14000rpm 10min 4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓遠心後、上清を捨て、</span><span lang="EN-US">70</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">％</span><span lang="EN-US">ethanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">200</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">加え、</span><span lang="EN-US">15000rpm 5min 4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓遠心後、上清を捨て乾燥させる。乾燥後</span><span lang="EN-US">milliQ44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を加えて</span><span lang="EN-US">voltex</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し、</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">中に</span><span lang="EN-US">DNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を溶解させる。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">下記の反応液を調整する</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">反応液調整（</span><span lang="EN-US">DIAP2</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物</span><span lang="EN-US"> in milliQ<span style="mso-spacerun: yes">&nbsp;
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</span>44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>10x M Buffer<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;</span>5</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>Xba 1<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp; </span>1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">反応液調整（</span><span lang="EN-US">vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>pUAST-flag vector in milliQ<span style="mso-spacerun: yes">&nbsp; </span>44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>10x H Buffer<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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</span>5</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>Xba 1<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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</span>1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓反応液調整後、</span><span lang="EN-US">37</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で</span><span lang="EN-US">20</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">時間静置</span></p>
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<p><span style="FONT-FAMILY: 'Century','serif'; FONT-SIZE: 10.5pt; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 11.0pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: EN-US; mso-fareast-language: JA; mso-bidi-language: AR-SA" lang="EN-US"><br style="PAGE-BREAK-BEFORE: always; mso-special-character: line-break" clear="all"></span>&nbsp;</p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt; mso-pagination: widow-orphan" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">8/31(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">水</span><span lang="EN-US">)</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">武田、横井川</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【目的】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">制限酵素処理した</span><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物・</span><span lang="EN-US">vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">のゲル抽出</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【実験操作】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">～反応液の電気泳動～</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">SeaKem<sup>R</sup>GTG<sup>R</sup></span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">のアガロースを使用して</span><span lang="EN-US">1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">％ゲルを作成した。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓制限酵素処理した反応液</span><span lang="EN-US">50</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">に対して</span><span lang="EN-US">6x loading dye</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">10</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を加えて混ぜ、アガロースゲルのコーム穴に入れた。（</span><span lang="EN-US">DNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">マーカーも同様に入れる）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">50V60min</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">で電気泳動した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓電気泳動後、</span><span lang="EN-US">EtBr</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">でゲルを染色した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓染色後、</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">でゲルを数回洗った。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">UV</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">イルミネーター上にゲルを置いたプレートをセットし、</span><span lang="EN-US">UV</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を点灯させてナイフで切り出した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓ゲルの重さを測定</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">～</span><span lang="EN-US">DNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">精製（</span><span lang="EN-US">QIAquick Gel Extraction kit</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を使用）～</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">ゲルの重さに対して三倍量の</span><span lang="EN-US">BufferQG</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を加え、</span><span lang="EN-US">50</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃のウォーターバスで</span><span lang="EN-US">incubate(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">約</span><span lang="EN-US">10min)</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">isopropanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">をゲルの重さと等量加えて混ぜた。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓精製カラムに溶液をセットし遠心した。下段に排出された溶液は廃棄。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">BufferQG500</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">をカラムにセットし遠心した。下段に排出された溶液は<br>
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&nbsp;
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廃棄。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">BufferPE750</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">をカラムにセットし遠心した。下段に排出された溶液は<br>
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&nbsp;
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廃棄。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓カラム本体を新しいチューブにセット、</span><span lang="EN-US">milliQ37</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を加えて遠心した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span>
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<span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">下段の新しいチューブに精製された</span><span lang="EN-US">DNA in milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">約</span><span lang="EN-US">35</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">ができた。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">～</span><span lang="EN-US">DNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">濃度の測定～</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'Arial','sans-serif'; COLOR: black; mso-bidi-font-size: 10.5pt" lang="EN-US">milliQ 100 &micro;L</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Arial; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial">および、精製した</span><span style="FONT-FAMILY: 'Arial','sans-serif'; COLOR: black; mso-bidi-font-size: 10.5pt" lang="EN-US">DNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Arial; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial">溶液を</span><span style="FONT-FAMILY: 'Arial','sans-serif'; COLOR: black; mso-bidi-font-size: 10.5pt" lang="EN-US">100</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Arial; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial">倍希釈した溶液を準備した。</span><span style="FONT-FAMILY: 'Arial','sans-serif'; COLOR: black; mso-bidi-font-size: 10.5pt" lang="EN-US"><br>
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</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Arial; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial">↓</span><span style="FONT-FAMILY: 'Arial','sans-serif'; COLOR: black; mso-bidi-font-size: 10.5pt" lang="EN-US">milliQ 100 &micro;L</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Arial; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial">でゼロ補正を行い、サンプル</span><span style="FONT-FAMILY: 'Arial','sans-serif'; COLOR: black; mso-bidi-font-size: 10.5pt" lang="EN-US">1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Arial; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial">、</span><span style="FONT-FAMILY: 'Arial','sans-serif'; COLOR: black; mso-bidi-font-size: 10.5pt" lang="EN-US">5</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Arial; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial">の吸光度を測定した。</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt; mso-pagination: widow-orphan" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【結果】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">pUAST-flag vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">は精製でき、濃度は</span><span lang="EN-US">45.0375ng/</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">であった。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">DIAP2</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">はゲル抽出でバンドが見られなかった。</span></p>
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<p><span style="FONT-FAMILY: 'Century','serif'; FONT-SIZE: 10.5pt; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 11.0pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: EN-US; mso-fareast-language: JA; mso-bidi-language: AR-SA" lang="EN-US"><br style="PAGE-BREAK-BEFORE: always; mso-special-character: line-break" clear="all"></span>&nbsp;</p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt; mso-pagination: widow-orphan" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">9/1(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">木</span><span lang="EN-US">)</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">武田</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【目的】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">cDNA vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">を鋳型とした</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">DIAP2</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">の増殖</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">（</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">）</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">反応液の調製</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN"><br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &lt;1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">本分あたり</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">&gt;<br>
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</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">　　</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">KOD+ polymelase</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">
+
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</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">　&nbsp;&nbsp;&nbsp;
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</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">&nbsp; 1 &micro;L<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 10 x
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Buffer</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">　</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2
+
-
&micro;L</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">　　　　　　</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN"><br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; dNTP(2.0
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&micro;M)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;5
+
-
&micro;L<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;F primer(10
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-
&micro;M)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+
-
1.5 &micro;L<br>
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-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; R primer(10
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-
&micro;M)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+
-
1.5
+
-
&micro;L&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br>
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-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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Templet
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-
DNA&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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1 &micro;L<br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+
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milliQ&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">&nbsp;33 &micro;L<o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">上記の分量を混合した。</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">(2)PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">反応条件</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN"><br>
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</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">　　</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">&lt;1&gt;×1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">　　　</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">Pre-denature&nbsp; </span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">　</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN"> 94 </span><span style="FONT-FAMILY: 'ＭＳ ゴシック'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: 'ＭＳ ゴシック'; mso-ansi-language: EN" lang="EN">℃</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">　　　</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">2 min<br>
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-
&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp; &lt;2&gt;×35</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">　&nbsp;　</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">Denature&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">　</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">&nbsp;94 </span><span style="FONT-FAMILY: 'ＭＳ ゴシック'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: 'ＭＳ ゴシック'; mso-ansi-language: EN" lang="EN">℃</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">　　</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">&nbsp;&nbsp; &nbsp;15
+
-
sec<br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;
+
-
Annealing&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;50.5 </span><span style="FONT-FAMILY: 'ＭＳ ゴシック'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: 'ＭＳ ゴシック'; mso-ansi-language: EN" lang="EN">℃</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">　　</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">&nbsp;&nbsp; 30
+
-
sec<br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+
-
&nbsp;&nbsp;
+
-
Extension&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;68
+
-
</span><span style="FONT-FAMILY: 'ＭＳ ゴシック'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: 'ＭＳ ゴシック'; mso-ansi-language: EN" lang="EN">℃</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+
-
&nbsp;1 min 40 sec<br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+
-
&nbsp;&nbsp;&lt;3&gt;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+
-
</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">　</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">4</span><span style="FONT-FAMILY: 'ＭＳ ゴシック'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: 'ＭＳ ゴシック'; mso-ansi-language: EN" lang="EN">℃</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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-
∞<br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">上記の条件で</span><span style="COLOR: black; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">を行った。</span><span style="COLOR: black; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-theme-font: minor-fareast; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-fareast; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">【目的】<span lang="EN"><br>
+
-
</span>　<span lang="EN">PCR</span>産物の確認<span lang="EN"><br>
+
-
</span>【実験操作】<span lang="EN"><br>
+
-
&nbsp;&nbsp;PCR</span>反応液　　　<span lang="EN">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;2 &micro;L<br>
+
-
&nbsp;
+
-
milliQ&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+
-
8 &micro;L<br>
+
-
&nbsp; 6 x loading dye&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 2
+
-
&micro;L<br>
+
-
&nbsp; </span>上記の溶液を混合し、この溶液を<span lang="EN">1%</span>アガロースゲルで<span lang="EN">100 V</span>、<span lang="EN">30</span>分の条件で電気泳動し<span lang="EN"><o:p></o:p></span></span></p>
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-
<p style="TEXT-ALIGN: left; TEXT-INDENT: 10.5pt; MARGIN: 0mm 0mm 0pt; mso-char-indent-count: 1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-theme-font: minor-fareast; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-fareast; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">た。泳動後、<span lang="EN">EtBr</span>染色を行い、写真を撮った。<span lang="EN"><o:p></o:p></span></span></p>
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<p><span style="FONT-FAMILY: 'Century','serif'; COLOR: black; FONT-SIZE: 10.5pt; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Arial; mso-bidi-font-family: Arial; mso-ansi-language: EN; mso-fareast-language: JA; mso-bidi-language: AR-SA" lang="EN"><br style="PAGE-BREAK-BEFORE: always; mso-special-character: line-break" clear="all"></span>&nbsp;</p>
+
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt; mso-pagination: widow-orphan" class="MsoNormal" align="left"><span style="COLOR: black; mso-hansi-font-family: Arial; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN"><o:p>&nbsp;</o:p></span></p>
+
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">9/5(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">月</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">)<o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">横井川</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p></o:p></span></p>
+
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【目的】</span></p>
+
-
<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物と</span><span lang="EN-US">pUAST-flag vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">の制限酵素処理（一回目：</span><span lang="EN-US">Xho 1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
+
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【実験操作】</span></p>
+
-
<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">～フェノールクロロホルム処理・エタノール沈殿～</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">DAIP2</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">の</span><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物を</span><span lang="EN-US">400</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">まで</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">で希釈し、フェノール・クロロホルムを</span><span lang="EN-US">400</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">加えて</span><span lang="EN-US">voltex </span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し</span><span lang="EN-US">10000rpm3min4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓水層のみを新しいチューブに移した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓クロロホルムを等量加えて、</span><span lang="EN-US">voltex</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し</span><span lang="EN-US">10000rpm3min4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓水層のみを新しいチューブに移し</span><span lang="EN-US">3M CH<sub>3</sub>COONa(PH 5.27)</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">1/10</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">等量加え、</span><span lang="EN-US">isopropanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を等量加えた。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">3</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">分静置後、</span><span lang="EN-US">14000rpm 10min 4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓遠心後、上清を捨て、</span><span lang="EN-US">70</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">％</span><span lang="EN-US">ethanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">200</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">加え、</span><span lang="EN-US">15000rpm 5min 4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で<br>
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&nbsp;
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遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓遠心後、上清を捨て乾燥させる。乾燥後</span><span lang="EN-US">milliQ44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を加えて</span><span lang="EN-US">voltex</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し、</span><span lang="EN-US">milliQ<br>
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&nbsp;&nbsp;&nbsp; </span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">中に</span><span lang="EN-US">DNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を溶解させる。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">～制限酵素処理～</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">フェノールクロロホルム処理後下記の反応液を調整した</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">反応液調整（</span><span lang="EN-US">cDNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物</span><span lang="EN-US"> in milliQ<span style="mso-spacerun: yes">&nbsp;&nbsp;
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</span>44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>10x H Buffer</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">　　　　</span><span lang="EN-US">5</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>Xho
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1<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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</span>1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓反応液調整後、</span><span lang="EN-US">37</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で</span><span lang="EN-US">20</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">時間静置</span></p>
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<p><span style="FONT-FAMILY: 'Century','serif'; FONT-SIZE: 10.5pt; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 11.0pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: EN-US; mso-fareast-language: JA; mso-bidi-language: AR-SA" lang="EN-US"><br style="PAGE-BREAK-BEFORE: always; mso-special-character: line-break" clear="all"></span>&nbsp;</p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt; mso-pagination: widow-orphan" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">9/6(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">火</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">)<o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">横井川</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">【目的】</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物と</span><span lang="EN-US">pUAST-flag vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">の制限酵素処理</span><span lang="EN-US">(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">二回目：</span><span lang="EN-US">Xbal)</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【実験操作】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">制限酵素処理</span><span lang="EN-US">20h</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">後、フェノールクロロホルム処理を行う</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物を</span><span lang="EN-US">400</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">まで</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">で希釈し、フェノール・クロロホルムを</span><span lang="EN-US">400</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">加えて</span><span lang="EN-US">voltex </span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し</span><span lang="EN-US">10000rpm3min4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓水層のみを新しいチューブに移した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓クロロホルムを等量加えて、</span><span lang="EN-US">voltex</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し</span><span lang="EN-US">10000rpm3min4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓水層のみを新しいチューブに移し</span><span lang="EN-US">3M CH<sub>3</sub>COONa(PH 5.27)</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">1/10</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">等量加え、</span><span lang="EN-US">isopropanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を等量加えた。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">3</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">分静置後、</span><span lang="EN-US">14000rpm 10min 4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓遠心後、上清を捨て、</span><span lang="EN-US">70</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">％</span><span lang="EN-US">ethanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">200</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">加え、</span><span lang="EN-US">15000rpm 5min 4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で<br>
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&nbsp;
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遠心。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓遠心後、上清を捨て乾燥させる。乾燥後</span><span lang="EN-US">milliQ44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を加えて</span><span lang="EN-US">voltex</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し、</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">中に</span><span lang="EN-US">DNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を溶解させる。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">下記の反応液を調整する</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">反応液調整（</span><span lang="EN-US">DIAP2</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物</span><span lang="EN-US"> in milliQ<span style="mso-spacerun: yes">&nbsp;
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</span>44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>10x M Buffer<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;</span>5</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>Xba 1<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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</span>1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">反応液調整（</span><span lang="EN-US">vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>pUAST-flag vector in milliQ<span style="mso-spacerun: yes">&nbsp; </span>44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>10x H Buffer<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp; </span>5</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>Xba 1<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓反応液調整後、</span><span lang="EN-US">37</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で</span><span lang="EN-US">20</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">時間静置</span></p>
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<p><span style="FONT-FAMILY: 'Century','serif'; FONT-SIZE: 10.5pt; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 11.0pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: EN-US; mso-fareast-language: JA; mso-bidi-language: AR-SA" lang="EN-US"><br style="PAGE-BREAK-BEFORE: always; mso-special-character: line-break" clear="all"></span>&nbsp;</p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt; mso-pagination: widow-orphan" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">9/7(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">水</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">)<o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">横井川</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【目的】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">制限酵素処理した</span><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物・</span><span lang="EN-US">vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">のゲル抽出</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【実験操作】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">～反応液の電気泳動～</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">SeaKem<sup>R</sup>GTG<sup>R</sup></span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">のアガロースを使用して</span><span lang="EN-US">1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">％ゲルを作成した。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓制限酵素処理した反応液</span><span lang="EN-US">50</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">に対して</span><span lang="EN-US">6x loading dye</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">10</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を加えて混ぜ、アガロースゲルのコーム穴に入れた。（</span><span lang="EN-US">DNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">マーカーも同様に入れる）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">50V60min</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">で電気泳動した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓電気泳動後、</span><span lang="EN-US">EtBr</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">でゲルを染色した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓染色後、</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">でゲルを数回洗った。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">UV</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">イルミネーター上にゲルを置いたプレートをセットし、</span><span lang="EN-US">UV</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">ランプを当ててバンドを確認した。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【結果】</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">バンドが確認できなかったためゲル抽出を行う事が出来なかった。</span></p>
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<p><span style="FONT-FAMILY: 'Century','serif'; FONT-SIZE: 10.5pt; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 11.0pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: EN-US; mso-fareast-language: JA; mso-bidi-language: AR-SA" lang="EN-US"><br style="PAGE-BREAK-BEFORE: always; mso-special-character: line-break" clear="all"></span>&nbsp;</p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt; mso-pagination: widow-orphan" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">9/13(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">火</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">)<o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">松浪、横井川</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【目的】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物と</span><span lang="EN-US">pUAST-flag vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">の制限酵素処理（一回目：</span><span lang="EN-US">Xho 1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【実験操作】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">～フェノールクロロホルム処理・エタノール沈殿～</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">DAIP2</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">の</span><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物を</span><span lang="EN-US">400</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">まで</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">で希釈し、フェノール・クロロホルムを</span><span lang="EN-US">400</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">加えて</span><span lang="EN-US">voltex </span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し</span><span lang="EN-US">10000rpm3min4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓水層のみを新しいチューブに移した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓クロロホルムを等量加えて、</span><span lang="EN-US">voltex</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し</span><span lang="EN-US">10000rpm3min4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓水層のみを新しいチューブに移し</span><span lang="EN-US">3M CH<sub>3</sub>COONa(PH 5.27)</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">1/10</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">等量加え、</span><span lang="EN-US">isopropanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を等量加えた。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">3</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">分静置後、</span><span lang="EN-US">14000rpm 10min 4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓遠心後、上清を捨て、</span><span lang="EN-US">70</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">％</span><span lang="EN-US">ethanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">200</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">加え、</span><span lang="EN-US">15000rpm 5min 4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓遠心後、上清を捨て乾燥させる。乾燥後</span><span lang="EN-US">milliQ44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を加えて</span><span lang="EN-US">voltex</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し、</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">中に</span><span lang="EN-US">DNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を溶解させる。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">～制限酵素処理～</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">フェノールクロロホルム処理後下記の反応液を調整した</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">反応液調整（</span><span lang="EN-US">cDNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物</span><span lang="EN-US"> in milliQ <span style="mso-spacerun: yes">&nbsp;
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</span>44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>10x H Buffer</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">　　　　</span><span lang="EN-US">5</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>Xho 1<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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</span>1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">　　反応液調整（</span><span lang="EN-US">vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>pUAST-flag
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vector(500ng/</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l)<span style="mso-spacerun: yes">&nbsp; </span>10</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>milliQ<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;</span>34</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>10x H
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Buffer<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>5</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>Xho 1<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓反応液調整後、</span><span lang="EN-US">37</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で</span><span lang="EN-US">20</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">時間静置</span></p>
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<p><span style="FONT-FAMILY: 'Century','serif'; FONT-SIZE: 10.5pt; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 11.0pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: EN-US; mso-fareast-language: JA; mso-bidi-language: AR-SA" lang="EN-US"><br style="PAGE-BREAK-BEFORE: always; mso-special-character: line-break" clear="all"></span>&nbsp;</p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt; mso-pagination: widow-orphan" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">9/14(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">水</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">)<o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">松浪、横井川</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【目的】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物と</span><span lang="EN-US">pUAST-flag vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">の制限酵素処理</span><span lang="EN-US">(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">二回目：</span><span lang="EN-US">Xbal)</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【実験操作】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">制限酵素処理</span><span lang="EN-US">20h</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">後、フェノールクロロホルム処理を行う</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物を</span><span lang="EN-US">400</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">まで</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">で希釈し、フェノール・クロロホルムを</span><span lang="EN-US">400</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">加えて</span><span lang="EN-US">voltex </span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し</span><span lang="EN-US">10000rpm3min4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓水層のみを新しいチューブに移した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓クロロホルムを等量加えて、</span><span lang="EN-US">voltex</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し</span><span lang="EN-US">10000rpm3min4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓水層のみを新しいチューブに移し</span><span lang="EN-US">3M CH<sub>3</sub>COONa(PH 5.27)</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">1/10</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">等量加え、</span><span lang="EN-US">isopropanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を等量加えた。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">3</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">分静置後、</span><span lang="EN-US">14000rpm 10min 4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓遠心後、上清を捨て、</span><span lang="EN-US">70</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">％</span><span lang="EN-US">ethanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">200</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">加え、</span><span lang="EN-US">15000rpm 5min 4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓遠心後、上清を捨て乾燥させる。乾燥後</span><span lang="EN-US">milliQ44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を加えて</span><span lang="EN-US">voltex</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し、</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">中に</span><span lang="EN-US">DNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を溶解させる。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">下記の反応液を調整する</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">反応液調整（</span><span lang="EN-US">DIAP2</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物</span><span lang="EN-US"> in milliQ<span style="mso-spacerun: yes">&nbsp;
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</span>44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>10x M Buffer<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp; </span>5</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>Xba 1<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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</span>1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><o:p>&nbsp; </o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">反応液調整（</span><span lang="EN-US">vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>pUAST-flag vector in milliQ<span style="mso-spacerun: yes">&nbsp; </span>44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>10x H Buffer<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;</span>5</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>Xba 1<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp;&nbsp; </span>1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓反応液調整後、</span><span lang="EN-US">37</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で</span><span lang="EN-US">20</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">時間静置</span></p>
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<p><span style="FONT-FAMILY: 'Century','serif'; FONT-SIZE: 10.5pt; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 11.0pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: EN-US; mso-fareast-language: JA; mso-bidi-language: AR-SA" lang="EN-US"><br style="PAGE-BREAK-BEFORE: always; mso-special-character: line-break" clear="all"></span>&nbsp;</p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt; mso-pagination: widow-orphan" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">9/15(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">木</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">)<o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">松浪、横井川</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【目的】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">制限酵素処理した</span><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物・</span><span lang="EN-US">vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">のゲル抽出</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【実験操作】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">～反応液の電気泳動～</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">SeaKem<sup>R</sup>GTG<sup>R</sup></span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">のアガロースを使用して</span><span lang="EN-US">1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">％ゲルを作成した。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓制限酵素処理した反応液</span><span lang="EN-US">50</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">に対して</span><span lang="EN-US">6x loading dye</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">10</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を加えて混ぜ、アガロースゲルのコーム穴に入れた。（</span><span lang="EN-US">DNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">マーカーも同様に入れる）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">50V60min</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">で電気泳動した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓電気泳動後、</span><span lang="EN-US">EtBr</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">でゲルを染色した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓染色後、</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">でゲルを数回洗った。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">UV</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">イルミネーター上にゲルを置いたプレートをセットし、</span><span lang="EN-US">UV</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">ランプを当ててバンドを確認した。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【結果】</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">バンドが確認できなかったためゲル抽出を行う事が出来なかった。</span></p>
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<p><span style="FONT-FAMILY: 'Century','serif'; FONT-SIZE: 10.5pt; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 11.0pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: EN-US; mso-fareast-language: JA; mso-bidi-language: AR-SA" lang="EN-US"><br style="PAGE-BREAK-BEFORE: always; mso-special-character: line-break" clear="all"></span>&nbsp;</p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt; mso-pagination: widow-orphan" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">9/17(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">土</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">)<o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">横井川</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【目的】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物と</span><span lang="EN-US">pUAST-flag vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">の制限酵素処理</span><span lang="EN-US">(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">二回目：</span><span lang="EN-US">Xbal)</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【実験操作】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">下記の反応液を調整する</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">反応液調整（</span><span lang="EN-US">DIAP2</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物</span>
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<span lang="EN-US">in TE<span style="mso-spacerun: yes">&nbsp;&nbsp;
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&nbsp;&nbsp;</span><span style="mso-spacerun: yes">&nbsp;&nbsp;</span>44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>10x M Buffer<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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</span>5</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt 21pt; mso-para-margin-left: 0gd" class="MsoListParagraph" align="left"><span lang="EN-US"><span style="mso-spacerun: yes">&nbsp; </span>Xba 1<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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</span>1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓反応液調整後、</span><span lang="EN-US">37</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で</span><span lang="EN-US">20</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">時間静置</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p><span style="FONT-FAMILY: 'Century','serif'; FONT-SIZE: 10.5pt; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: EN-US; mso-fareast-language: JA; mso-bidi-language: AR-SA" lang="EN-US"><br style="PAGE-BREAK-BEFORE: always; mso-special-character: line-break" clear="all"></span>&nbsp;</p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt; mso-pagination: widow-orphan" class="MsoNormal" align="left"><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">9/18(</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">日</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">)<o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">横井川</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">【目的】</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="mso-bidi-font-size: 10.5pt" lang="EN-US">LB</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">培地づくり</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 10.5pt">【実験操作】</span><span style="mso-bidi-font-size: 10.5pt" lang="EN-US"><o:p></o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-theme-font: minor-fareast; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-fareast; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">↓LB</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-theme-font: minor-fareast; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-fareast; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">培地用の試薬を計り、ビーカーに入れる<span lang="EN"><o:p></o:p></span></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-theme-font: minor-fareast; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-fareast; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">↓950 ml</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-theme-font: minor-fareast; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-fareast; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">の蒸留水を入れ、スターラーで混ぜて溶かす<span lang="EN"><o:p></o:p></span></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-theme-font: minor-fareast; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-fareast; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">↓</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-theme-font: minor-fareast; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-fareast; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">三角フラスコに分注し、アルミ箔で二重にフタをして<span lang="EN"><o:p></o:p></span></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-theme-font: minor-fareast; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-fareast; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN">↓121°C</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-theme-font: minor-fareast; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-fareast; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN">で<span lang="EN">20</span>分オートクレーブ滅菌する<span lang="EN"><o:p></o:p></span></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; COLOR: black; mso-ascii-theme-font: minor-fareast; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-fareast; mso-bidi-font-size: 10.5pt; mso-bidi-font-family: Arial; mso-ansi-language: EN" lang="EN"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【目的】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">制限酵素処理した</span><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物・</span><span lang="EN-US">vector</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">のゲル抽出</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【実験操作】</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">制限酵素処理</span><span lang="EN-US">20h</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">後、フェノールクロロホルム処理を行う</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">PCR</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">産物を</span><span lang="EN-US">400</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">まで</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">で希釈し、フェノール・クロロホルムを</span><span lang="EN-US">400</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">加えて</span><span lang="EN-US">voltex </span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し</span><span lang="EN-US">10000rpm3min4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓水層のみを新しいチューブに移した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓クロロホルムを等量加えて、</span><span lang="EN-US">voltex</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し</span><span lang="EN-US">10000rpm3min4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓水層のみを新しいチューブに移し</span><span lang="EN-US">3M CH<sub>3</sub>COONa(PH 5.27)</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">1/10</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">等量加え、</span><span lang="EN-US">isopropanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を等量加えた。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">3</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">分静置後、</span><span lang="EN-US">14000rpm 10min 4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓遠心後、上清を捨て、</span><span lang="EN-US">70</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">％</span><span lang="EN-US">ethanol</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">200</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">加え、</span><span lang="EN-US">15000rpm 5min 4</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">℃で遠心。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓遠心後、上清を捨て乾燥させる。乾燥後</span><span lang="EN-US">milliQ44</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を加えて</span><span lang="EN-US">voltex</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">し、</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">中に</span><span lang="EN-US">DNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を溶解させる。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">～反応液の電気泳動～</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span lang="EN-US">SeaKem<sup>R</sup>GTG<sup>R</sup></span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">のアガロースを使用して</span><span lang="EN-US">1</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">％ゲルを作成した。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓制限酵素処理した反応液</span><span lang="EN-US">50</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">に対して</span><span lang="EN-US">6x loading dye</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を</span><span lang="EN-US">10</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">μ</span><span lang="EN-US">l</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">を加えて混ぜ、アガロースゲルのコーム穴に入れた。（</span><span lang="EN-US">DNA</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">マーカーも同様に入れる）</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">50V60min</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">で電気泳動した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓電気泳動後、</span><span lang="EN-US">EtBr</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">でゲルを染色した。</span></p>
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<p style="TEXT-ALIGN: left; MARGIN: 0mm 0mm 0pt" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓染色後、</span><span lang="EN-US">milliQ</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">でゲルを数回洗った。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">↓</span><span lang="EN-US">UV</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">イルミネーター上にゲルを置いたプレートをセットし、</span><span lang="EN-US">UV</span><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">ランプを当ててバンドを確認した。</span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
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<p style="TEXT-ALIGN: left; TEXT-INDENT: -10.5pt; MARGIN: 0mm 0mm 0pt 10.5pt; mso-char-indent-count: -1.0" class="MsoNormal" align="left"><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'ＭＳ 明朝'; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin">【結果】</span></p>
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<p><span style="FONT-FAMILY: 'ＭＳ 明朝','serif'; FONT-SIZE: 10.5pt; mso-ascii-font-family: Century; mso-ascii-theme-font: minor-latin; mso-fareast-theme-font: minor-fareast; mso-hansi-font-family: Century; mso-hansi-theme-font: minor-latin; mso-bidi-font-size: 11.0pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: EN-US; mso-fareast-language: JA; mso-bidi-language: AR-SA">バンドが確認できなかったためゲル抽出を行う事が出来なかった。</span></font></p>
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Revision as of 11:18, 25 September 2011

Retrieved from "http://2011.igem.org/Team:KIT-Kyoto/yokoigawa"