Team:USC/Notebook/Week5

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Revision as of 05:43, 25 September 2011

Official Team Profile

Week 5:

07/05/2011
1. digestion step by step for MET25 plasmid see protocols in general protocols

07/06/2011
1. Purify Gel DNA
2. Run a gell test :
MET25, pRS423-426, tetO+cFP, tetO+RFP, tetO+GFP,CAS3, CASO 3. PCR CASO primers

07/07/2011
1. Transformation for all 4 ligation plasmids:
TPS1, MSN2, ELO1, OLE1+pRS vectors
2. Gel verify for CASO
3. Amplify the verified products by PCR
4. Reisolate the genomic DNA
5. PCR CASO by using old primers