Team:USC/Notebook/Week13

From 2011.igem.org

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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 1:'''</h3>
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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 13:'''</h3>
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<span style="font-family:Verdana; font-weight:700;"> 06/07/2011 </span>
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<span style="font-family:Verdana; font-weight:700;"> 09/13/2011 </span>
<br />
<br />
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Transformation plasmids into DH5α Competent cells
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1. Check quality of gDNA
<br />
<br />
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Plasmids are from iGEM plates:
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2. Make HT115 + BL21 (tetO, CRISPR-GFP #7) competent cells, freeze stock
<br />
<br />
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A. Plate 2 Well 7E  (AHL signaling)
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3. Miniprep PCDF+casO
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<br />
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B. Plate 3 Well 20F  (LovTAP composite)
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<br />
<br />
C. Plate 4 Well 16O  (LovTAP Composite)
C. Plate 4 Well 16O  (LovTAP Composite)
<br />
<br />
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D. Plate 1 Well 2B  (GFP+PEST191)
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4. Digest tetO/tetR
<br />
<br />
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E. Plate 2 Well 4A  (Yeast ADH1 promoter)
 
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<span style="font-family:Verdana; font-weight:700;"> 06/08/2011 </span>
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<span style="font-family:Verdana; font-weight:700;"> 09/15/2011 </span>
<br />
<br />
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Transformation of E. Coli, Plasmids are from iGEM plates:
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1. Run a gel of all minipreped PCDF+casO<br />
<br />
<br />
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Plasmids are from iGEM plates:
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2. PCR HT115, DH5α2, DH5α3 with primers<br />
<br />
<br />
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A. Plate 2 Well 13J
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<span style="font-family:Verdana; font-weight:700;"> 09/18/2011 </span>
<br />
<br />
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B. Plate 1 Well 20F
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1. Inoculate the following:
<br />
<br />
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C. Plate 4 Well 16M
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    BL21 tetO::GFP
<br />
<br />
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D. Plate 1 Well 16K
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2. BL21 tetO::GFP, CRISPR::GFP#7
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3. BL21 tetO::GFP, CRISPR::GFP#7, cas3
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4. HT115 tetO::GFP
 +
5. HT115 tetO::GFP, CRISPR::GFP#7
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6. HT115 tetO::GFP, CRISPR::GFP#7, cas3
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7. HT115 CRISPR::GFP #7
<br />
<br />
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E. Plate 3 Well 14H
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C. Plate 4 Well 16O  (LovTAP Composite)
<br />
<br />
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F. Plate 4 Well 6O
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D. Plate 1 Well 2B  (GFP+PEST191)
<br />
<br />
 +
E. Plate 2 Well 4A  (Yeast ADH1 promoter)
 +
 +
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<span style="font-family:Verdana; font-weight:700;"> 06/09/2011 </span>
 
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<br />
 
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1. Inoculation for all the plasmid we have transformed before.
 
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Observation: all transformation except BBa-I15010
 
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<br />
 
<span style="font-family:Verdana; font-weight:700;"> 06/10/2011 </span>
<span style="font-family:Verdana; font-weight:700;"> 06/10/2011 </span>

Revision as of 05:26, 25 September 2011

USC-logo.jpg IGEM2011-logo.jpg IGEM-logo.jpg
USC Banner.jpg

Brainstorming
Brain storm.JPG

Week 1
Week1.jpg

Week 2
Week2.jpg

Week 3
Week3.jpg

Week 4
Week4.jpg

Week 5
Week5.jpg

Week 6
Week6.jpg

Week 7
Week7.jpg

Week 8
Week8.jpg

Week 9
Week9.jpg

Week 10
Week10.jpg

Week 11
Week11.jpg

Week 12
Week12.jpg

Week 13
Week13.jpg

Week 13:

09/13/2011
1. Check quality of gDNA
2. Make HT115 + BL21 (tetO, CRISPR-GFP #7) competent cells, freeze stock
3. Miniprep PCDF+casO
C. Plate 4 Well 16O (LovTAP Composite)
4. Digest tetO/tetR

09/15/2011
1. Run a gel of all minipreped PCDF+casO

2. PCR HT115, DH5α2, DH5α3 with primers

09/18/2011
1. Inoculate the following: 

   BL21 tetO::GFP


2. BL21 tetO::GFP, CRISPR::GFP#7 3. BL21 tetO::GFP, CRISPR::GFP#7, cas3 4. HT115 tetO::GFP 5. HT115 tetO::GFP, CRISPR::GFP#7 6. HT115 tetO::GFP, CRISPR::GFP#7, cas3 7. HT115 CRISPR::GFP #7
C. Plate 4 Well 16O (LovTAP Composite)
D. Plate 1 Well 2B (GFP+PEST191)
E. Plate 2 Well 4A (Yeast ADH1 promoter)



06/10/2011
1. Freeze the inoculated culture
2. Observation: BBa-I15008 and BBa-I63009don’t have as much growth as others

06/11/2011
1. Purify plasmid DNA from the culture

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