Team:USC/Notebook/Week3

From 2011.igem.org

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Revision as of 05:25, 25 September 2011

Official Team Profile

Week 3:

06/20/2011
1. Lab meeting
2. Ligate the blunted ended DNA

06/21/2011
1. Column purify the DNA
2. Make buffers for competent cells
Buffer: 10mM KOAc+80mM CaCl2-2H2O+70mM MnCl2-4H2O+10mM MgCl2-6H2O
3. Transformation again for:
Plasmids:OLE1, MSN2, TSP1, ELO1, CAS0, pSB1AK8
Vectors: prs 423-426

06/22/2011
1. Inoculation for :
pRS vectors 423-426 and pSB1AK8
2. PCR ligation products:
OLE1, MSN2, TSP1, ELO1

06/23/2011
1. Transfer E.Coli from agar stabs from iGEM delivery
BBa-K191005
BBa-K191004
BBa-K426020
BBa-I15010
Notes:
+ The agar should already have a hole from when it was stabbed. With an inoculating loop dipped into the stab, you can plate directly
onto a petri dish with the appropriate antibiotic;
+ Incubate the dish overnight at 37 C (14-16hrs)
+Pick a single colony to start up a culture
2. Gel running to test OLE1, MSN2, TSP1, ELO1, CAS0,
Observation: OLE was the only one with a clear band

@@ Line 164: / Line 164: @@
 <span style="font-family:Verdana; font-weight:700;"> 06/22/2011 </span>
 <br />
-.	1.	Inoculation for :
+.	Inoculation for :
 <br />
 pRS vectors 423-426 and pSB1AK8
@@ Line 171: / Line 171: @@
 <br />
 OLE1, MSN2, TSP1, ELO1
+<br />
+<span style="font-family:Verdana; font-weight:700;"> 06/23/2011 </span>
+<br />
+.	Transfer E.Coli from agar stabs from iGEM delivery
+<br />
+BBa-K191005
+<br />
+BBa-K191004
+<br />
+BBa-K426020
+<br />
+BBa-I15010
+<br />
+Notes:
+<br />
++ The agar should already have a hole from when it was stabbed. With an inoculating loop dipped into the stab, you can plate directly
+<br />
+onto a petri dish with the appropriate antibiotic;
+<br />
++ Incubate the dish overnight at 37 C (14-16hrs)
+<br />
++Pick a single colony to start up a culture
+<br />
+.	Gel running to test OLE1, MSN2, TSP1, ELO1, CAS0,
+<br />
+Observation: OLE was the only one with a clear band
 <br />
 </table>