Team:UC Davis/LacI

From 2011.igem.org

(Difference between revisions)
Line 21: Line 21:
</div>
</div>
-
<div id="mutantwidget" class="floatbox2">
 
-
<h3>Wildtype</h3>
 
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 
-
<span class="ydata">
 
-
0.99315,
 
-
  0.78019, 
 
-
  0.58405,
 
-
  0.45400, 
 
-
  0.35401,
 
-
  0.29099, 
 
-
  0.13305, 
 
-
</span>
 
-
<span class="numconditions">4</span>
 
-
<span class='xdata_3d'>0, 1, 2, 5</span>
 
-
<span class='ydata_3d'>0.00000,  0.20000,  0.40000,  0.60000,  0.80000,  1.00000,  2.00000</span>
 
-
<span class='zdata_3d'>
 
-
  0.99315,  1.02097,  0.99023,  0.99354,
 
-
  0.78019,  0.97021,  0.96725,  0.96226,
 
-
  0.58405,  0.87100,  0.89795,  0.94529,
 
-
  0.45400,  0.76333,  0.85071,  0.93618,
 
-
  0.35401,  0.65243,  0.75444,  0.88056,
 
-
  0.29099,  0.55454,  0.68266,  0.84441,
 
-
  0.13305,  0.26829,  0.37015,  0.55294</span>
 
-
<h3>Mutant 1</h3>
 
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 
-
<span class="ydata">
 
-
  0.68829,
 
-
  0.48655, 
 
-
  0.27198,
 
-
  0.15260, 
 
-
  0.08426,
 
-
  0.05322, 
 
-
  0.01741, 
 
-
</span>
 
-
<h3>Mutant 2</h3>
 
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
 
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 
-
<span class="ydata">
 
-
  0.68514,
 
-
  0.43727, 
 
-
  0.27724,
 
-
  0.17817, 
 
-
  0.11684,
 
-
  0.08021, 
 
-
  0.03415, 
 
-
</span>
 
-
<h3>Mutant 3</h3>
 
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 
-
<span class="ydata">
 
-
  0.67026,
 
-
  0.44322, 
 
-
  0.28504,
 
-
  0.17543, 
 
-
  0.10938,
 
-
  0.07325, 
 
-
  0.02937, 
 
-
</span>
 
-
<h3>Mutant 4</h3>
 
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
 
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 
-
<span class="ydata">
 
-
  0.31888,
 
-
  0.16625, 
 
-
  0.10228,
 
-
  0.05821, 
 
-
  0.03985,
 
-
  0.02549, 
 
-
  0.01329, 
 
-
</span>
 
-
<h3>Mutant 5</h3>
 
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 
-
<span class="ydata">
 
-
  0.63174,
 
-
  0.33524, 
 
-
  0.17440,
 
-
  0.09337, 
 
-
  0.03043,
 
-
  0.03337, 
 
-
  0.01686, 
 
-
</span>
 
-
<h3>Mutant 6</h3>
 
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 
-
<span class="ydata">
 
-
  0.42290,
 
-
  0.29347, 
 
-
  0.15733,
 
-
  0.09471, 
 
-
  0.05778,
 
-
  0.03850, 
 
-
  0.01475, 
 
-
</span>
 
-
<h3>Mutant 7</h3>
 
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca</span>
 
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 
-
<span class="ydata">
 
-
  0.58989,
 
-
  0.32402, 
 
-
  0.20047,
 
-
  0.11183, 
 
-
  0.06365,
 
-
  0.03915, 
 
-
  0.01712, 
 
-
</span>
 
-
 
-
 
-
</div>
 

Revision as of 00:47, 25 September 2011

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Criteria

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LacI

The lac repressor is responsible for regulating the metabolism of lactose. In the absence of lactose, LacI forms a tetramer with identical subunits which appears as two dimers. Each dimer binds in the major groove of the DNA binding region which subsequently blocks the RNA polymerase from binding. In nature, allolactose will bind the repressor leading to transcription of the lac operon. Using IPTG as an inducer has the same effect as allolactose.

3D model

Construct

DNA Sequence

Retrieved from "http://2011.igem.org/Team:UC_Davis/LacI"