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Team:Grinnell/Recipes
From 2011.igem.org
(Difference between revisions)
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<th>Color Code</th> | <th>Color Code</th> | ||
<th>Stock (mg/mL)</th> | <th>Stock (mg/mL)</th> | ||
| - | <th>LB Plate | + | <th>LB Plate Conc (μg/mL)</th> |
| - | <th>PYE Plate | + | <th>PYE Plate Conc (μg/mL)</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Ampicillin (amp)</td> | + | <td style="text-align:left">Ampicillin (amp)</td> |
<td>Red</td> | <td>Red</td> | ||
<td>50</td> | <td>50</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Carbenicillin (carb)</td> | + | <td style="text-align:left">Carbenicillin (carb)</td> |
<td>Red</td> | <td>Red</td> | ||
<td>50</td> | <td>50</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Chloramphenicol (chlor)</td> | + | <td style="text-align:left">Chloramphenicol (chlor)</td> |
<td>2 Orange</td> | <td>2 Orange</td> | ||
<td>5 (in EtOH)</td> | <td>5 (in EtOH)</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Kanamycin (kan)</td> | + | <td style="text-align:left">Kanamycin (kan)</td> |
<td>blue</td> | <td>blue</td> | ||
<td>50</td> | <td>50</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Nalidixic acid (nal)</td> | + | <td style="text-align:left">Nalidixic acid (nal)</td> |
<td>Green</td> | <td>Green</td> | ||
<td>10</td> | <td>10</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Tetracycline (tet)</td> | + | <td style="text-align:left">Tetracycline (tet)</td> |
<td>Black</td> | <td>Black</td> | ||
<td>2 (in EtOH)</td> | <td>2 (in EtOH)</td> | ||
<td>12</td> | <td>12</td> | ||
| - | <td> | + | <td>–</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Oxytetracycline (oxytet)</td> | + | <td style="text-align:left">Oxytetracycline (oxytet)</td> |
<td>Black</td> | <td>Black</td> | ||
<td>2 (in EtOH)</td> | <td>2 (in EtOH)</td> | ||
| - | <td> | + | <td>−</td> |
<td>2</td> | <td>2</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Spectinomycin (spec)</td> | + | <td style="text-align:left">Spectinomycin (spec)</td> |
<td>Purple</td> | <td>Purple</td> | ||
<td>20</td> | <td>20</td> | ||
Revision as of 22:31, 21 June 2011
L Broth
For 1L:- 10g Tryptone
- 5g Yeast extract
- 5g NaCl
PYE Broth
For 1L:- 2g Bacto peptone
- 1g Yeast extract
- 1mL 1M MgSO4⋅7H2O
- 1mL 0.5M CaCl2
Agar Plates
Add 15g Bacto-agar to 1L of liquid media before autoclaving.Antibiotics Kitchen
| Antibiotic | Color Code | Stock (mg/mL) | LB Plate Conc (μg/mL) | PYE Plate Conc (μg/mL) |
|---|---|---|---|---|
| Ampicillin (amp) | Red | 50 | 100 | 50 |
| Carbenicillin (carb) | Red | 50 | 50 | 50 |
| Chloramphenicol (chlor) | 2 Orange | 5 (in EtOH) | 30 | 1 |
| Kanamycin (kan) | blue | 50 | 50 | 25 |
| Nalidixic acid (nal) | Green | 10 | 20 | 20 |
| Tetracycline (tet) | Black | 2 (in EtOH) | 12 | – |
| Oxytetracycline (oxytet) | Black | 2 (in EtOH) | − | 2 |
| Spectinomycin (spec) | Purple | 20 | 50 | 100 |
Note: for liquid cultures in PYE broth, use half the concentration that would be used for a PYE plate (LB remains the same for liquid and sold cultures).
