Team:Caltech/Recipes

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Caltech iGEM 2011



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Agar/LB plate:

1) Fill two bottles with 500 g of nanopure water, 10 g of Tryptone, 10 g of NaCl, 5 g of yeast extract, and 15 g of Agar.

2) Shake and then autoclave.

50% Glycerol Stock:
1) Pour 50 mL of Glycerol and 50 mL of pure water into a graduated cylinder.
2) Cover with parafilm and turn over a couple of times to mix.
3) Use the Nalgene vacuum to sterilize the stock.
4) Use the bunsen burner to keep air sterile and close the cap.

Enrichment Minimal Media

  • 1.0712 g of K2 HPO4
  • 0.5239 g of KH2 PO4
  • 79 mL of Phosphate solution
  • 100 mL of Salt Solution I
  • 100 mL of Salt Solution II
  • 10 mL of Wolfe's Vitamin Solution
  • 1 mL of SL-10 Trace Element Solution.

and then add 5 mg of whatever chemical used in two flasks, one with vitamins and one without.


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