27 (June 27, 2011 GDS)
From 2011.igem.org
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Revision as of 15:23, 24 September 2011
Safety:
Initial Bleach Kill Curve
Sample Bacteria for OD and initial cell number
- -log phase culture OD= .194
- -stationary phase culture OD= .685
- - No cell count for either (too many)
Experiment
-Mix 4.5 mL culture with 500λ bleach
-Incubate for 15 minutes and plate 1 mL
-Collect 1 mL and spin to pellet
-Wash twice with TE and resuspend in 100λ LB and then plate
- - Result: No Colonies
Note: LB plates had bright pink colonies