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Revision as of 15:12, 24 September 2011 (view source) Sallen (Talk | contribs) ← Older edit		Revision as of 15:12, 24 September 2011 (view source) Sallen (Talk | contribs) Newer edit →
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-	-Spin 1.5 mL of overnight culture in microfuge
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-	-Aspirate all but 100uL of the supernatant and resuspend pellet by vortexing
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-	-Add 300uL of TENS and mix by inversion
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-	-Add 150uL of sodium acetate and vortex
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-	-Centrifuge for 2.5 minutes at 10K</br>
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-	-Transfer supernatant to clean tube and add 1mL of room temp. ETOH
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-	-Mix and pellet DNA by centrifuge for 2-5 min. at 10K
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-	-Wash pellet with 70% ethanol and allow pellet to dry
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-	-Resuspend pellet in 30uL of TE w/ RNA seA
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-	-Digest 5-10uL as usual
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-	:To make TENS, add:
-	::-4.5mL of TE
-	:::-250uL 10%SDS
-	:::-250uL 2N NaOH
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	<gallery widths=910px heights=800px>		<gallery widths=910px heights=800px>
	File:June7GDS.jpg		File:June7GDS.jpg
	</gallery>		</gallery>

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Revision as of 15:12, 24 September 2011