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From 2011.igem.org

Revision as of 19:43, 5 September 2011 (view source) Sallen (Talk | contribs) ← Older edit		Revision as of 15:12, 24 September 2011 (view source) Sallen (Talk | contribs) Newer edit →
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	:::-250uL 10%SDS		:::-250uL 10%SDS
	:::-250uL 2N NaOH		:::-250uL 2N NaOH
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Revision as of 15:12, 24 September 2011

-Spin 1.5 mL of overnight culture in microfuge

-Aspirate all but 100uL of the supernatant and resuspend pellet by vortexing

-Add 300uL of TENS and mix by inversion

-Add 150uL of sodium acetate and vortex

-Centrifuge for 2.5 minutes at 10K</br>

-Transfer supernatant to clean tube and add 1mL of room temp. ETOH

-Mix and pellet DNA by centrifuge for 2-5 min. at 10K

-Wash pellet with 70% ethanol and allow pellet to dry

-Resuspend pellet in 30uL of TE w/ RNA seA

-Digest 5-10uL as usual

To make TENS, add:

-4.5mL of TE

-250uL 10%SDS

-250uL 2N NaOH