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Team:Kyoto/Protocol
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* corn flour : 45g | * corn flour : 45g | ||
* glucose : 50g | * glucose : 50g | ||
| + | * agarose : 3.5~5g | ||
| + | * propionic acid : 1.5mL | ||
| + | * 10% p-hydroxybenzoate in 70% Eternol : 5g | ||
| + | | | ||
| + | # Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it. | ||
| + | # Stir corn flour and glucose with the remaining water. | ||
| + | # Stir 1 and 2, then autoclave it again. | ||
| + | # after autoclave, add propionic acid and 10% p-hydroxybenzoate in 70% Eternol into it.■ | ||
| + | |} | ||
| + | <br/> | ||
| + | ===M9 medium=== | ||
| + | ::{| class="wikitable" | ||
| + | ! width="300" |Materials | ||
| + | ! width="300" |Methods | ||
| + | |- | ||
| + | | | ||
| + | * water : 1 L | ||
| + | * Na2HPO4: 6 g | ||
| + | * KH2PO4 : 3 g | ||
| + | * NaCl : 0.5 g | ||
| + | * NH4Cl : 1 g | ||
* agarose : 3.5~5g | * agarose : 3.5~5g | ||
* propionic acid : 1.5mL | * propionic acid : 1.5mL | ||
Revision as of 04:32, 23 September 2011
Protocol
Medium for drosophila
[http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Medium for drosophila] Materials Methods - water : 500mL
- dry yeast : 20g
- corn flour : 45g
- glucose : 50g
- agarose : 3.5~5g
- propionic acid : 1.5mL
- 10% p-hydroxybenzoate in 70% Eternol : 5g
- Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it.
- Stir corn flour and glucose with the remaining water.
- Stir 1 and 2, then autoclave it again.
- after autoclave, add propionic acid and 10% p-hydroxybenzoate in 70% Eternol into it.■
M9 medium
Materials Methods - water : 1 L
- Na2HPO4: 6 g
- KH2PO4 : 3 g
- NaCl : 0.5 g
- NH4Cl : 1 g
- agarose : 3.5~5g
- propionic acid : 1.5mL
- 10% p-hydroxybenzoate in 70% Eternol : 5g
- Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it.
- Stir corn flour and glucose with the remaining water.
- Stir 1 and 2, then autoclave it again.
- after autoclave, add propionic acid and 10% p-hydroxybenzoate in 70% Eternol into it.■
