Team:UC Davis/LacI

From 2011.igem.org

(Difference between revisions)
Line 22: Line 22:
<h3>Wildtype</h3>
<h3>Wildtype</h3>
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
0.99315,
 +
  0.78019, 
 +
  0.58405,
 +
  0.45400, 
 +
  0.35401,
 +
  0.29099, 
 +
  0.13305, 
 +
</span>
 +
<span class="numconditions">4</span>
<h3>Mutant 1</h3>
<h3>Mutant 1</h3>
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.68829,
 +
  0.48655, 
 +
  0.27198,
 +
  0.15260, 
 +
  0.08426,
 +
  0.05322, 
 +
  0.01741, 
 +
</span>
<h3>Mutant 2</h3>
<h3>Mutant 2</h3>
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.68514,
 +
  0.43727, 
 +
  0.27724,
 +
  0.17817, 
 +
  0.11684,
 +
  0.08021, 
 +
  0.03415, 
 +
</span>
<h3>Mutant 3</h3>
<h3>Mutant 3</h3>
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.67026,
 +
  0.44322, 
 +
  0.28504,
 +
  0.17543, 
 +
  0.10938,
 +
  0.07325, 
 +
  0.02937, 
 +
</span>
<h3>Mutant 4</h3>
<h3>Mutant 4</h3>
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.31888,
 +
  0.16625, 
 +
  0.10228,
 +
  0.05821, 
 +
  0.03985,
 +
  0.02549, 
 +
  0.01329, 
 +
</span>
<h3>Mutant 5</h3>
<h3>Mutant 5</h3>
<span class="sequence">caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
<span class="sequence">caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.63174,
 +
  0.33524, 
 +
  0.17440,
 +
  0.09337, 
 +
  0.03043,
 +
  0.03337, 
 +
  0.01686, 
 +
</span>
<h3>Mutant 6</h3>
<h3>Mutant 6</h3>
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.42290,
 +
  0.29347, 
 +
  0.15733,
 +
  0.09471, 
 +
  0.05778,
 +
  0.03850, 
 +
  0.01475, 
 +
</span>
<h3>Mutant 7</h3>
<h3>Mutant 7</h3>
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca</span>
+
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.58989,
 +
  0.32402, 
 +
  0.20047,
 +
  0.11183, 
 +
  0.06365,
 +
  0.03915, 
 +
  0.01712, 
 +
</span>
</div>
</div>
 +
<div class="floatbox2">
<div class="floatbox2">

Revision as of 23:43, 22 September 2011

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Criteria

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LacI

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Wildtype

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.99315, 0.78019, 0.58405, 0.45400, 0.35401, 0.29099, 0.13305, 4

Mutant 1

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.68829, 0.48655, 0.27198, 0.15260, 0.08426, 0.05322, 0.01741,

Mutant 2

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.68514, 0.43727, 0.27724, 0.17817, 0.11684, 0.08021, 0.03415,

Mutant 3

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.67026, 0.44322, 0.28504, 0.17543, 0.10938, 0.07325, 0.02937,

Mutant 4

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.31888, 0.16625, 0.10228, 0.05821, 0.03985, 0.02549, 0.01329,

Mutant 5

caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.63174, 0.33524, 0.17440, 0.09337, 0.03043, 0.03337, 0.01686,

Mutant 6

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.42290, 0.29347, 0.15733, 0.09471, 0.05778, 0.03850, 0.01475,

Mutant 7

caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.58989, 0.32402, 0.20047, 0.11183, 0.06365, 0.03915, 0.01712,

3D model

Construct

DNA Sequence

Retrieved from "http://2011.igem.org/Team:UC_Davis/LacI"