Team:Johns Hopkins/Modeling/FullVitCLBS

The Full Pathway+Expression L-Ascorbate Model in LBS

directive sample 90000.0 5000
 
// Compartments
comp cytosol = new comp;
comp nucleus = new comp inside cytosol;
 
// Transcriptional machinery
spec Ribo = new{mrna:binding};
spec RNAP = new{dna:binding,mrna:binding};
 
// Gene-Enzyme Pairs
spec GME = new{bind:binding}; spec sub_GDP_D_mannose_epimerase 
= new{}; spec GDP_D_mannose_epimerase = new{};
spec VTC5 = new{bind:binding}; spec GDP_L_Gal_phosphorylase = new{};
spec ARA2 = new{bind:binding}; spec D_Ara_dehydrogenase = new{};
spec ALO1 = new{bind:binding}; spec D_Ara_Lac_oxidase = new{};
spec VTC4 = new{bind:binding}; spec Gal_P_phosphatase = new{};
spec VTC2 = new{bind:binding}; spec GDP = new{};
 
// Metabolites
spec GDP_D_Man = new{};
spec GDP_L_Gal = new{};
spec L_Gal_P = new{};
spec L_Gal = new{};
spec L_Gal_Lac = new{};
spec L_ascorbate = new{};
 
// Rate constants
rate GDP_clearance = 1.0E-9;
rate kb = 0.01;
rate ku = 1.0E-4;
rate GDP_D_mannose_epimerase_Km = 0.0045;
rate GDP_D_mannose_epimerase_Kcat = 0.041;
rate Ki = 5.0e-4;
rate GDP_L_Gal_phosphorylase_Km = 0.01;
rate GDP_L_Gal_phosphorylase_Kcat = 13.0;
rate Gal_P_phosphatase_Km = 0.107;
rate Gal_P_phosphatase_Kcat = 6.0;
rate D_Ara_dehydrogenase_Km = 180.0;
rate D_Ara_dehydrogenase_Kcat = 125.0;
rate L_Gal_Lac_dehydrogenase_Km = 53.7;
rate L_Gal_Lac_dehydrogenase_Kcat = 18.8;
 
//Transcription Kinetics
rate rna_deg = 0.0058;
rate rna_falloff = 0.01;
rate rnap_bind = 0.5;
rate transcription = 10;
rate termination = 10;
rate ribo_bind = 0.1;
rate ribo_unbind = 0.01;
rate translation = 10;
rate export = 0.01;
rate prot_deg = 0.01;
 
module transcribe(spec DNA:{bind}, mRNA:{down}) {
	RNAP{dna} + DNA{bind} ->{rnap_bind} RNAP{dna!1}-DNA{bind!1} |
	RNAP{dna!1}-DNA{bind!1} ->{transcription} mRNA{down!2}-RNAP{dna!1,mrna!2}-DNA{bind!1} |
	mRNA{down!2}-RNAP{dna!1,mrna!2}-DNA{bind!1} ->{termination} mRNA{down} + RNAP{dna,mrna} + 
DNA{bind}
};
 
module translate(spec mRNA:{up}, Prot) {
	Ribo{mrna} + mRNA{up} <->{ribo_bind}{ribo_unbind} Ribo{mrna!1}-mRNA{up!1} |
	Ribo{mrna!1}-mRNA{up!1} ->{translation} Ribo{mrna} + mRNA{up} + Prot
};
 
module express(comp nuc; spec DNA:{bind}, Prot) {
	spec mRNA = new{up:binding,down:binding};
	nuc[ transcribe(DNA:{bind}, mRNA:{down}) | mRNA ->{rna_deg} ] |
	nuc[ mRNA ] ->{export} mRNA |
	translate(mRNA:{up}, Prot) | mRNA -> {rna_deg}
};
 
module mmInhibitionRXN(spec sub, enz, prod, inhib; rate km, kcat, k) {
	rate rxn_rate = kcat*enz/(sub+km*(1+inhib/k));
	sub + enz ->[rxn_rate] enz + prod
};
 
module mmRXN(spec sub, enz, prod; rate km, kcat) {
	rate rxn_rate = kcat*enz/(sub+km);
	sub + enz ->[rxn_rate] enz + prod
};
 
module mmTwoProductsRXN(spec sub, enz, prod1, prod2; rate km, kcat) {
	rate rxn_rate = kcat*enz/(sub+km);
	sub + enz ->[rxn_rate] enz + prod1 + prod2
};
 
cytosol[
	express(nucleus, GME:{bind}, sub_GDP_D_mannose_epimerase) |
	express(nucleus, VTC5:{bind}, GDP_L_Gal_phosphorylase) |
	express(nucleus, VTC2:{bind}, GDP) |
	express(nucleus, VTC4:{bind}, Gal_P_phosphatase) |
	express(nucleus, ARA2:{bind}, D_Ara_dehydrogenase) |
	express(nucleus, ALO1:{bind}, D_Ara_Lac_oxidase) |
	sub_GDP_D_mannose_epimerase + sub_GDP_D_mannose_epimerase <->{kb}{ku} 
GDP_D_mannose_epimerase |
	mmInhibitionRXN(GDP_D_Man, GDP_D_mannose_epimerase, GDP_L_Gal, GDP, 
GDP_D_mannose_epimerase_Km, GDP_D_mannose_epimerase_Kcat, Ki) |
	mmTwoProductsRXN(GDP_L_Gal, GDP_L_Gal_phosphorylase, L_Gal_P, GDP, 
GDP_L_Gal_phosphorylase_Km, GDP_L_Gal_phosphorylase_Kcat) |
	mmRXN(L_Gal_P, Gal_P_phosphatase, L_Gal, Gal_P_phosphatase_Km, Gal_P_phosphatase_Kcat) |
	mmRXN(L_Gal, D_Ara_dehydrogenase, L_Gal_Lac, D_Ara_dehydrogenase_Km, 
D_Ara_dehydrogenase_Kcat) |
	mmRXN(L_Gal_Lac, D_Ara_Lac_oxidase, L_ascorbate, L_Gal_Lac_dehydrogenase_Km, 
L_Gal_Lac_dehydrogenase_Kcat) |
		->{10} GDP_D_Man |
		GDP ->{GDP_clearance} |
		sub_GDP_D_mannose_epimerase ->{prot_deg} |
		GDP_D_mannose_epimerase ->{prot_deg} |
		GDP_L_Gal_phosphorylase ->{prot_deg} |
		D_Ara_dehydrogenase ->{prot_deg} |
		D_Ara_Lac_oxidase ->{prot_deg} |
		Gal_P_phosphatase ->{prot_deg} |
		init Ribo 10 |
	nucleus[
		init GME 1 |
		init VTC2 1 |
		init VTC4 1 |
		init ARA2 1 |
		init VTC5 1 |
		init ALO1 1 |
		init RNAP 1
	]
]