Results

Gel photo after PCR cloning of the individual gyrase binding sites

The three bands below the 400 bp mark on the gel photo indicate successful PCR of our gyrase binding sites

Chloroquine gel displaying the difference in plasmid supercoiling between control and the three GBS

This gel clearly displays the superior property of the Mu GBS in introducing negative supercoils into a plasmid, as the bands for this plasmid travelled the farthest from the well. Although the pSC101 counterpart did manage to improve the supercoiling to a similar extent, the quality and consistency was still below that for Mu GBS. On the other hand the pBR322 GBS proved to be worse at introducing supercoils into the plasmid when compared to the control (pSB1C3 with RFP coding insert).