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Team:Paris Bettencourt/Data
From 2011.igem.org
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# '''SinOp reporter strain''', given by J.W. Veening, published in this paper [http://jb.asm.org/cgi/content/full/192/8/2053/DC1] | # '''SinOp reporter strain''', given by J.W. Veening, published in this paper [http://jb.asm.org/cgi/content/full/192/8/2053/DC1] | ||
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<h2>The lambda switch system</h2> | <h2>The lambda switch system</h2> | ||
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<h3>Data for our favorite new parts</h3> | <h3>Data for our favorite new parts</h3> | ||
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| - | # [http://partsregistry.org/Part: | + | # [http://partsregistry.org/Part:BBa_K606044 Main Page] - '''pVeg SpoVg CI TT, BBa_K606044''': Expression casette for for CI in B. subtilis. |
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| + | <h3>Data for Pre-existing Parts</h3> | ||
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| + | # [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - '''pVeg + SpoVG, BBa_K143053''' - (Imperial College, iGEM 2008) | ||
| + | # [http://partsregistry.org/Part:BBa_B0015 Main Page] - '''Double standard terminator, BBa_B0015''' - Standard double terminator. | ||
| + | # [http://partsregistry.org/Part:BBa_C0052 Main Page] - '''CI, BBa_B0052''' - CI gene from the lambda phage. | ||
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<h2>GFP diffusion</h2> | <h2>GFP diffusion</h2> | ||
Revision as of 02:51, 22 September 2011
Data page
The YFP concentration system
Data for our favorite new parts
- [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
Data for Pre-existing Parts
- [http://partsregistry.org/Part:BBa_K090403:Experience Experience] - Gram positive chromosomal integration plasmid, BBa_K090403 (Cambridge, iGEM 2008):
The T7 polymerase diffusion system
Data for our favorite new parts
- [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
- [http://partsregistry.org/Part:BBa_K606003 Main Page] - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
- [http://partsregistry.org/Part:BBa_K606025 Main Page] - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
Data for Pre-existing Parts
- [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):
The tRNA Amber diffusion system
Data for our favorite new parts
- [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
- [http://partsregistry.org/Part:BBa_K606003 Main Page] - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
- [http://partsregistry.org/Part:BBa_K606025 Main Page] - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
Data for Pre-existing Parts
- [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):
The ComS diffusion system
Data for our favorite new parts
- [http://partsregistry.org/Part:BBa_K606037 Main Page] - ComS gene, BBa_K606037: The comS gene can trigger the MecA/ComS/ComK system. The start and stop codon has been changed for more standard codons.
- [http://partsregistry.org/Part:BBa_K606038 Main Page] - ComS gene Codon optimized, BBa_K606038: This is the same gene than the previous one, but it has been synthetized de novo.
Data for Pre-existing Parts
- [http://partsregistry.org/Part:BBa_K143053 Main Page] - pVeg + SpoVG, BBa_K143053 promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
- [http://partsregistry.org/Part:BBa_B0015 Main Page] - Double standard terminator, BBa_B0015 standard double terminator
- [http://partsregistry.org/Part:BBa_K143021 Main Page] - RFP, BBa_K143021 RFP m-cherry.
- Elowitz's pComG-CFP/pComS-YFP reporter strain - Competence reporter strain. Published in this paper [http://www.elowitz.caltech.edu/publications/Tunability.pdf]
- CodY- strain - From Abraham L. Sonenshein's laboratory, Tufts university [http://www.tufts.edu/sackler//facultyIntros/sonensheinA.html]
The SinOp system
Data for our favorite new parts
- [http://partsregistry.org/Part:BBa_K606046 Main Page] - KinA gene, BBa_K606046 KinA gene. SinOp kinase.
- [http://partsregistry.org/Part:BBa_K606047 Main Page] - pVeg-SpoVg-KinA gene, BBa_K606047 KinA gene with B. subtilis promoter
Data for Pre-existing Parts
- SinOp reporter strain, given by J.W. Veening, published in this paper [http://jb.asm.org/cgi/content/full/192/8/2053/DC1]
The lambda switch system
Data for our favorite new parts
- [http://partsregistry.org/Part:BBa_K606044 Main Page] - pVeg SpoVg CI TT, BBa_K606044: Expression casette for for CI in B. subtilis.
Data for Pre-existing Parts
- [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 - (Imperial College, iGEM 2008)
- [http://partsregistry.org/Part:BBa_B0015 Main Page] - Double standard terminator, BBa_B0015 - Standard double terminator.
- [http://partsregistry.org/Part:BBa_C0052 Main Page] - CI, BBa_B0052 - CI gene from the lambda phage.
GFP diffusion
Data for Pre-existing Parts
- [http://partsregistry.org/Part:BBa_K143079 Experience] - Pveg-spoVG GFP expression construct, BBa_K143079 (Imperial College, iGEM 2008):
