Team:Paris Bettencourt/Data

From 2011.igem.org

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<h2>The ComS diffusion system</h2>
<h2>The ComS diffusion system</h2>
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<h3>Data for our favorite new parts</h3>
<h3>Data for our favorite new parts</h3>
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# [http://partsregistry.org/Part:BBa_K606026 Main Page] - '''TetO Array, BBa_K606026''': 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
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# [http://partsregistry.org/Part:BBa_K606037 Main Page] - '''ComS gene, BBa_K606037''': The comS gene can trigger the MecA/ComS/ComK system. The start and stop codon has been changed for more standard codons.
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# [http://partsregistry.org/Part:BBa_K606003 Main Page] - '''Multihost replicative plasmid pHM3, BBa_K606003''': This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
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# [http://partsregistry.org/Part:BBa_K606038 Main Page] - '''ComS gene Codon optimized, BBa_K606038''': This is the same gene than the previous one, but it has been synthetized de novo.
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# [http://partsregistry.org/Part:BBa_K606025 Main Page] - '''YFP:tetR recombinant fusion protein, BBa_K606025''': This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
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<h3>Data for Pre-existing Parts</h3>
<h3>Data for Pre-existing Parts</h3>
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# [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - '''pVeg + SpoVG, BBa_K143053''' (Imperial College, iGEM 2008):  
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# [http://partsregistry.org/Part:BBa_K143053 Main Page] - '''pVeg + SpoVG, BBa_K143053''' promoter and RBS for B. subtilis (Imperial College, iGEM 2008)  
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# [http://partsregistry.org/Part:BBa_B0015 Main Page] - '''Double standard terminator, BBa_B0015''' standard double terminator
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# [http://partsregistry.org/Part:BBa_K143021 Main Page] - '''RFP, BBa_K143021''' RFP m-cherry.
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# Elowitz's pComG-CFP/pComS-YFP reporter strain - Competence reporter strain. Published in this paper [http://www.elowitz.caltech.edu/publications/Tunability.pdf]
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# CodY- strain - From Abraham L. Sonenshein's laboratory, Tufts university [http://www.tufts.edu/sackler//facultyIntros/sonensheinA.html]
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<h2>The KinA system</h2>
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<h2>The SinOp system</h2>
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<p>To be completed</p>
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<img src=https://static.igem.org/mediawiki/2011/5/5f/SinOp.004.png width=600px>
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<h3>Data for our favorite new parts</h3>
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</html>
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# [http://partsregistry.org/Part:BBa_K606046 Main Page] - '''KinA gene, BBa_K606046''' KinA gene. SinOp kinase.
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# [http://partsregistry.org/Part:BBa_K606047 Main Page] - '''pVeg-SpoVg-KinA gene, BBa_K606047''' KinA gene with B. subtilis promoter
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<html>
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<h3>Data for Pre-existing Parts</h3>
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</html>
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# SinOp reporter strain, given by J.W. Veening, published in this paper [http://jb.asm.org/cgi/content/full/192/8/2053/DC1]
<h2>The lambda switch system</h2>
<h2>The lambda switch system</h2>

Revision as of 02:41, 22 September 2011

Team IGEM Paris 2011

Data page

The YFP concentration system

The YFP concentration system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K090403:Experience Experience] - Gram positive chromosomal integration plasmid, BBa_K090403 (Cambridge, iGEM 2008):



The T7 polymerase diffusion system

The T7 polymerase diffusion system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. [http://partsregistry.org/Part:BBa_K606003 Main Page] - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. [http://partsregistry.org/Part:BBa_K606025 Main Page] - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



The tRNA Amber diffusion system

The tRNA Amber diffusion system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. [http://partsregistry.org/Part:BBa_K606003 Main Page] - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. [http://partsregistry.org/Part:BBa_K606025 Main Page] - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



The ComS diffusion system

The ComS diffusion system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606037 Main Page] - ComS gene, BBa_K606037: The comS gene can trigger the MecA/ComS/ComK system. The start and stop codon has been changed for more standard codons.
  2. [http://partsregistry.org/Part:BBa_K606038 Main Page] - ComS gene Codon optimized, BBa_K606038: This is the same gene than the previous one, but it has been synthetized de novo.

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053 Main Page] - pVeg + SpoVG, BBa_K143053 promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
  2. [http://partsregistry.org/Part:BBa_B0015 Main Page] - Double standard terminator, BBa_B0015 standard double terminator
  3. [http://partsregistry.org/Part:BBa_K143021 Main Page] - RFP, BBa_K143021 RFP m-cherry.
  4. Elowitz's pComG-CFP/pComS-YFP reporter strain - Competence reporter strain. Published in this paper [http://www.elowitz.caltech.edu/publications/Tunability.pdf]
  5. CodY- strain - From Abraham L. Sonenshein's laboratory, Tufts university [http://www.tufts.edu/sackler//facultyIntros/sonensheinA.html]

The SinOp system

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606046 Main Page] - KinA gene, BBa_K606046 KinA gene. SinOp kinase.
  2. [http://partsregistry.org/Part:BBa_K606047 Main Page] - pVeg-SpoVg-KinA gene, BBa_K606047 KinA gene with B. subtilis promoter

Data for Pre-existing Parts

  1. SinOp reporter strain, given by J.W. Veening, published in this paper [http://jb.asm.org/cgi/content/full/192/8/2053/DC1]

The lambda switch system

</html>

The lambda switch system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. [http://partsregistry.org/Part:BBa_K606003 Main Page] - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. [http://partsregistry.org/Part:BBa_K606025 Main Page] - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

GFP diffusion

Nanotube and GFP.jpeg

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143079 Experience] - Pveg-spoVG GFP expression construct, BBa_K143079 (Imperial College, iGEM 2008):



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