Team:Glasgow/BiofilmResults

From 2011.igem.org

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<h2>Summary</h2>
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<p>- New Chassis</p>
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<p>- Forms biofilms</p>
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<p>- Non-pathogenic and compatible with majority of biobricks</p>
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<p>-No shuttle vector necessary</p>
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<p>-Time series shows that biofilm grows at similar speed to planktonic cells</p>

Revision as of 01:01, 22 September 2011

Results

The images below show a selection of stages of biofilm formation. Starting with Image 1 showing a lab strain of E.colithat has no fimbriae, and is not forming a biofilm.

Image 2 shows an EM of E.coli Nissle 1917 in the early stages of biofilm formation. The fimbriae that allow the cells to cling to each other are clearly visible.

Image 3 shows a Nissle biofilm in the later stages of formation, with the cells densely packed and the extracellular matrix that holds them together showing.

Image 1: 15,000x EM of E.coli for comparison.
No fimbriae or EPS is visible.
(courtesy of Rocky Mountain Laboratories)

Picture 4: 10,000x SEM image of Nissle
showing the fimbriae

Image 3: SEM image of Nissle biofilm
showing the extracellular matrix

Image 1: 1000x EM of P. aeruginosa biofilm,
showing its densely packed structure
(courtesy of Dan Walker, University of Glasgow)


Summary

- New Chassis

- Forms biofilms

- Non-pathogenic and compatible with majority of biobricks

-No shuttle vector necessary

-Time series shows that biofilm grows at similar speed to planktonic cells