Team:Grenoble/Projet/Intro
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Revision as of 00:19, 22 September 2011
The Project, Mercuro-Coli
Introduction :
With industries growth, wastes are accumulating and presence of pollutant and toxic products is becoming an international concern.
Our project is oriented in this problematic, Mercuro-Coli is a mercury biosensor which allows to detect and quantify mercury contained into polluted water. Intended to fieldwork studies, the device should be very easy to used :
- Ideally, the device should be a plate carried into a packaging.
- Once unpacked, the sample just has to be added.
- Finally, the result would be given by a red strip : its presence testifying that the sample contains mercury and its position on the plate acquainting about the quantity.
Specifications :
At the beginning of the project, specifications were defined about the final device. To get a better understanding on how the device should run, those are going to be taken back step by step.
- Operating living cell : E. Coli: BW 25 113
- Only one type of bacteria :
Once our genetic circuit achieved, it will be introduce to our E. Coli strain. Newly obtained bacteria strain will be then spread on the plate forming a bacteria film on the top of it.
- Comparative measure :
By adding uniformly the polluted sample, two different bacteria behaviors will come up depending on the predominant concentration :
So, an IPTG gradient concentration will be made beforehand constituting our scale of quantification.
- A visual result :
The two different behaviors were chosen in purpose.
Introduction :
With industries growth, wastes are accumulating and presence of pollutant and toxic products is becoming an international concern.
Our project is oriented in this problematic, Mercuro-Coli is a mercury biosensor which allows to detect and quantify mercury contained into polluted water. Intended to fieldwork studies, the device should be very easy to used :
- Ideally, the device should be a plate carried into a packaging.
- Once unpacked, the sample just has to be added.
- Finally, the result would be given by a red strip : its presence testifying that the sample contains mercury and its position on the plate acquainting about the quantity.
Specifications :
At the beginning of the project, specifications were defined about the final device. To get a better understanding on how the device should run, those are going to be taken back step by step.
- Operating living cell : E. Coli: BW 25 113
- Only one type of bacteria :
Once our genetic circuit achieved, it will be introduce to our E. Coli strain. Newly obtained bacteria strain will be then spread on the plate forming a bacteria film on the top of it.
- Comparative measure :
By adding uniformly the polluted sample, two different bacteria behaviors will come up depending on the predominant concentration :
So, an IPTG gradient concentration will be made beforehand constituting our scale of quantification.
- A visual result :
The two different behaviors were chosen in purpose.