Team:Wageningen UR/Project/PartsProj1

From 2011.igem.org

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(→Synchroscillator: Data page/parts submitted to the registry)
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=== Data for our favorite new parts ===
=== Data for our favorite new parts ===
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[http://partsregistry.org/Part:BBa_K546546 BBa_K546546] - '''The Synchroscillator:''' Our goal was to achieve synchronous oscillatory behavior in a population of ''E. coli'' cells. Our data shows that this has been achieved. Moreover, we have created the system in such a way that it is theoretically tuneable in two ways.
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[http://partsregistry.org/Part:BBa_K546546 BBa_K546546] - '''The Synchroscillator:''' Our goal was to achieve synchronized oscillatory behavior in a population of ''E. coli'' cells. Our data shows that this has been achieved. Moreover, we have designed the system in such a way that its expression dynamics are tuneable when operated in conjunction with a tuner device.
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[http://partsregistry.org/Part:BBa_K546005 BBa_K546005] - '''Quorum-induced positive feedback loop quantifiable by GFP:''' AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of autoinducer synthetase. The system creates a positive feedback loop, yielding high titres of GFP.
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[http://partsregistry.org/Part:BBa_K546005 BBa_K546005] - '''AHL-induced AHL synthase generator with fluorescent reporter:''' Our data shows an increase in GFP expression per cell over time, providing evidence that the GFP expression is under regulation of a positive feedback loop. This is in accordance with our expectations.
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[http://partsregistry.org/Part:BBa_K546001 BBa_K546001] - '''3OC6HSL Receiver Device with a regulated 3OC6HSL inactivation enzyme gene:''' AHL responsive element that produces a low constitutive level of luxR via pL. Combined with 3-oxohexanoyl-homoserine lactone, luxR induces pR. lux pR regulates the autoinducer inactivation enzyme A (aiiA) gene that degrades AHL.
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[http://partsregistry.org/Part:BBa_K546001 BBa_K546001] - '''AHL-induced AHL hydrolase generator:''' From our data we can conclude that AHL-induced GFP expression significantly drops after treatment with AiiA in contrast to no treatment with AiiA. This is in accordance with our expectations.
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[http://partsregistry.org/Part:BBa_I763020 BBa_I763020] - '''GFP + LVA Tag''' Green fluorescent protein with a degradation tag fused that cares for a shorter lifetime of the protein.
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[http://partsregistry.org/Part:BBa_I763020 BBa_I763020] - '''GFP + LVA Tag''' GFP is expressed and the degradation time
=== Other new parts ===
=== Other new parts ===

Revision as of 23:56, 21 September 2011

Building a Synchronized Oscillatory System

Synchroscillator: Data page/parts submitted to the registry

Data for our favorite new parts

[http://partsregistry.org/Part:BBa_K546546 BBa_K546546] - The Synchroscillator: Our goal was to achieve synchronized oscillatory behavior in a population of E. coli cells. Our data shows that this has been achieved. Moreover, we have designed the system in such a way that its expression dynamics are tuneable when operated in conjunction with a tuner device.

[http://partsregistry.org/Part:BBa_K546005 BBa_K546005] - AHL-induced AHL synthase generator with fluorescent reporter: Our data shows an increase in GFP expression per cell over time, providing evidence that the GFP expression is under regulation of a positive feedback loop. This is in accordance with our expectations.

[http://partsregistry.org/Part:BBa_K546001 BBa_K546001] - AHL-induced AHL hydrolase generator: From our data we can conclude that AHL-induced GFP expression significantly drops after treatment with AiiA in contrast to no treatment with AiiA. This is in accordance with our expectations.


Data for pre-existing parts

[http://partsregistry.org/Part:BBa_I0460 BBa_I0460] - aiiA Device: Autoinducer inactivation enzyme A (AiiA) is capable of degrading the quorum sensing molecule AHL.


[http://partsregistry.org/Part:BBa_K082014 BBa_K082014] - Autoinducer synthethase: Produces the quorum sensing molecule 3-oxohexanoyl-homoserine lactone (AHL).


[http://partsregistry.org/Part:BBa_I763020 BBa_I763020] - GFP + LVA Tag GFP is expressed and the degradation time

Other new parts

[http://partsregistry.org/Part:BBa_K546002 BBa_K546002] - 3OC6HSL Receiver Device with a lux pR regulated GFP (including a LVA Tag) Reporter: AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of GFP. Upon addition of AHL the GFP expression increases.

[http://partsregistry.org/Part:BBa_K546000 BBa_K546000] - Autoinducer 3OC6HSL Receiver Device with a lux pR Controlled Autoinducer Synthetase: AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of autoinducer synthetase.

[http://partsregistry.org/Part:BBa_K546003 BBa_K546003] - 3OC6HSL Receiver Device with lux pR: 3-oxohexanoyl-homoserine lactone (AHL) responsive element that constitutively produces an intermediate level of LuxR via lux pL promoter. Combined with AHL, luxR activates lux pR promoter. This part is a repaired version of [http://partsregistry.org/Part:BBa_F2621 BBa_F2621] that appeared to be defective (R0063 missing), when we sequenced the part.

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