Revision as of 22:40, 21 September 2011

muTetRs

In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outline

First we chose the site-directed mutagenesis approach to make the mutants of TetR but that strategy did not work well

TetR variants	Mutagenesis	Promoter	Cterminal	DNA form	Expression	MITOMI vs 1-off	Biobrick	Sequenced	Sent to registry
V36F	site directed	SP6	eGFP	plasmid	worked well	X	BBa_K613013
V36FW43S	PCR-induced	T7	His-tag	linear	(ins C-term), ITT tested		BBa_K613014
E37AW43ST141A	PCR-induced	T7	His-tag	linear	ITT tested	X	BBa_K613015	X	X
P39K	PCR-induced	T7	His-tag	template	ITT tested, worked	X	BBa_K613016	X	X
Y42F	PCR-induced	T7	His-tag	linear	ITT tested	X	BBa_K613017	X	X
Y42FK108E	PCR-induced	T7	His-tag	linear	ITT tested		BBa_K613018	X	X
P39QY42M	site directed	SP6	eGFP	plasmid	worked well	X	BBa_K613019
P39QY42ML197S	PCR-induced	T7	His-tag	linear	ITT tested		BBa_K613020
P39QY42ML52P	PCR-induced	T7	His-tag	linear	ITT tested		BBa_K613021
E37AP39K	site directed	T7	His-tag	linear			BBa_K613022	X
E37AP39QY42F	site directed	T7	His-tag	linear			BBa_K613023	X
WT	none	T7	His&GFP	both

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	==How==		==How==

-	First we chose the site directed mutagenesis approach to make the mutants of TetR but that strategy <html> <a href="https://2011.igem.org/Team:EPF-Lausanne/Notebook/July2011#Tuesday.2C_12_July_2011"> did not work well </a> </html>	+	First we chose the site-directed mutagenesis approach to make the mutants of TetR but that strategy <html> <a href="https://2011.igem.org/Team:EPF-Lausanne/Notebook/July2011#Tuesday.2C_12_July_2011"> did not work well </a> </html>