Team:Freiburg/Notebook/8 September

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==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==
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===NAME OF YOUR EXPERIMENT===
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===Troubleshooting of the modified Lysis genes K124017===
-
 
+
-
'''Investigators:NAME'''
+
 +
'''Investigators:Theo'''
 +
Transformation of M66 with M48 did not work. Probably because the stock of M66 that we made was indeed another part.
 +
<br>
 +
So <ins>S4+S15 Nr1 is going to be '''Nr1'''</ins> and <ins>S4+S15 Nr7 is '''Nr7'''</ins>.
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<br>
 +
M48+1 and M48+2 as well as just Nr1 and Nr2 were inoculated so that they could be sent for sequencing on Friday
==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==

Revision as of 20:57, 21 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

Troubleshooting of the modified Lysis genes K124017

Investigators:Theo

Transformation of M66 with M48 did not work. Probably because the stock of M66 that we made was indeed another part.
So S4+S15 Nr1 is going to be Nr1 and S4+S15 Nr7 is Nr7.
M48+1 and M48+2 as well as just Nr1 and Nr2 were inoculated so that they could be sent for sequencing on Friday

Precipitator

Ligation

Name: Rüdiger Date: 08.09.
Continue from Date 05.09. Name Rüdiger

Experiment Digestion

Project Name: Precipitator

Procedure


PCR tube:

total volume 20 μl


  1. add H2O (17 μl -X-Y-Z)
  2. add 2 μl Ligase Buffer 10x
  3. add Insert 1, Insert 2(when proceeding from 3A digestion use 2 μl of each)
  4. add Vector (20ng needed. When proceeding from 3A digestion use 2 μl)
  5. Add 1 μl T4-DNA Ligase
  6. Incubate 10-30 min at room temperature
  7. heat for 20 minutes at 80°C
  8. store at -20°C or directly proceed to transformation


Name of part Ratio Insert:Vector

= 3:1 or 1:1

Volume (μl)
X insert 1
Y insert 2
Z vector
H2O

Documentation:

Why are you doing this experiment? Where are your parts stored? Name the parts for ligation etc.


Name Vector Insert
4a C3, 05.09. digest 4 05.09. digest
8a C3, 05.09. digest 8 05.09. digest
9a C3, 05.09. digest 9 05.09. digest
10a C3, 05.09. digest 10 05.09. digest
4b C3, 05.09. digest 4 31.08. digest
8b C3, 05.09. digest 8 31.08. digest
9b C3, 05.09. digest 9 31.08. digest
10b C3, 05.09. digest 10 31.08. digest



NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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