Team:Bilkent UNAM Turkey/Experiment

From 2011.igem.org

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We repeat this step a lot because of failuire.I put right result<br>
We repeat this step a lot because of failuire.I put right result<br>
<img src="https://static.igem.org/mediawiki/2011/3/3e/Restriction_products_of_minipreps_of_pRbcnfsI_unam.jpg" align="center"></img>
<img src="https://static.igem.org/mediawiki/2011/3/3e/Restriction_products_of_minipreps_of_pRbcnfsI_unam.jpg" align="center"></img>
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We used standard <a href="http://tools.invitrogen.com/content/sfs/manuals/purelink_pro96_plasmid_kit_man.pdf">gel purification kit</a>
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<br>We used standard <a href="http://tools.invitrogen.com/content/sfs/manuals/purelink_pro96_plasmid_kit_man.pdf">gel purification kit</a>
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Revision as of 20:13, 21 September 2011

We ordered our genes. We optimized codon usage because of gene taken from E. cloacae.

Codon Usage Then we did restriction digestion and gel electrophoresis.
We repeat this step a lot because of failuire.I put right result

We used standard gel purification kit