Team:ITESM Mexico/Parts submitted

From 2011.igem.org

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{{Team:ITESM_Mexico/Top}}
{{Team:ITESM_Mexico/Top}}
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Name     Description    Type     Designer         Length    Lenght (prefix;suffix)
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BBa_K587000 RecA Protein coding  David Maycotte/        1062         1103
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                        sequences        Patricia Campos
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BBa_K587001 Crxst Regulatory   David Maycotte   35         78
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BBa_K587002 Tast Regulatory   David Maycotte   80         123
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BBa_K587003 Tawk Regulatory   David Maycotte   79         122
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BBa_K587004 Crxwk Regulatory   David Maycotte   35         78
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BBa_K587005 Itast Regulatory   David Maycotte   81         124
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'''''*RecA'''''
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Sequence
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'''GAATTCGCGGCCGCTTCTAG'''ATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATTGAGAAACAATTTGGTAAAGGCTCCATCATGCGCCTGGGTGAAGACCGTTCCATGGATGTGGAAACCATCTCTACCGGTTCGCTTTCACTGGATATCGCGCTTGGGGCAGGTGGTCTGCCGATGGGCCGTATCGTCGAAATCTACGGACCAGAATCTTCCGGTAAAACCACGCTGACGTTGCAGGTGATCGCCGCAGCGCAGCGCGAAGGTAAAACCTGTGCGTTTATCGATGCTGAACACGCGCTGGACCCAATCTACGCACGTAAACTGGGCGTCGATATTGACAACCTGCTGTGCTCCCAGCCGGATACCGGCGAGCAGGCACTGGAAATCTGTGATGCCCTGGCACGTTCTGGCGCAGTAGACGTTATCGTCGTTGACTCCGTGGCGGCACTGACGCCGAAAGCGGAAATCGAAGGCGAAATCGGCGACTCTCACATGGGCCTTGCGGCACGTATGATGAGCCAGGCGATGCGTAAGCTGGCGGGTAACCTGAAGCAGTCCAACACGCTGCTGATCTTCATCAACCAGATCCGTATGAAAATTGGTGTGATGTTCGGTAACCCGGAAACCACTACCGGTGGTAACGCGCTGAAATTCTACGCCTCTGTTCGTCTCGACATCCGTCGTATCGGCGCGGTGAAAGAGGGCGAAAACGTGGTGGGTAGCGAAACCCGCGTGAAAGTGGTGAAGAACAAAATCGCTGCGCCGTTTAAACAGGCTGAGTTCCAGATCCTCTACGGCGAAGGTATCAACTTCTACGGCGAGCTGGTTGACCTGGGCGTGAAAGAGAAGCTTATCGAGAAAGCAGGCGCGTGGTACAGCTACAAAGGTGAGAAGATCGGTCAGGGTAAAGCGAATGCGACTGCCTGGCTGAAAGATAACCCGGAAACCGCGAAAGAGATCGAGAAGAAAGTACGTGAGTTGCTGCTGAGCAACCCGAACTCAACGCCGGATTTCTCTGTAGATGACAGCGAAGGCGTAGCAGAAACTAACGAAGATTTTTAA'''TACTAGTAGCGGCCGCTGCAG'''
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'''''*Crxst'''''
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This DNA sequence acts as a lock for the low arabinose concentration regulation path.
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Sequence
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'''GAATTCGCGGCCGCTTCTAGAG'''AAGCGCAGTAATTCACCTCTTGGATTTGGGTATCA'''TACTAGTAGCGGCCGCTGCAG'''
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'''''*Tast'''''
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This sequence acts as the key for the Crxst lock and it unlocks it, in order to activate following mechanism.
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Sequence
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'''GAATTCGCGGCCGCTTCTAGAG'''AGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGAACCTAAAGGGATAGTT
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'''TACTAGTAGCGGCCGCTGCAG'''
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'''''*Tawk'''''
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This sequence acts as the key fot Crxwk lock and it unlocks it, in order to activate the following mechanism.
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Sequence
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'''GAATTCGCGGCCGCTTCTAGAG'''AGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGATAAACCGTAAGGGTT
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'''TACTAGTAGCGGCCGCTGCAG'''
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'''''*Crxwk'''''
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This DNA sequence acts as a lock for the high arabinose concentration regulation path.
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Sequence
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'''GAATTCGCGGCCGCTTCTAGAG'''AAGCGCAGTAATTCACCTCTATTTGGCATTCCCAA'''TACTAGTAGCGGCCGCTGCAG'''
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'''''*Itast'''''
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This is a complementary sequence for the Tast key, so we call it the antikey since it will inactivate the Tast key and preventing it to interact with the Crxst lock.
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Sequence
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'''GAATTCGCGGCCGCTTCTAGAG'''TCTAGGAGAGATATATGGTATGGTGGTTAATTTTCATTAACCACCAAAGCGCAGTAATTCACCTCTTGGTTTCCCTATCAA
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'''TACTAGTAGCGGCCGCTGCAG'''

Revision as of 18:54, 21 September 2011

ITESM MÉXICO

SensE.coli

Igem Itesm

Name Description Type Designer Length Lenght (prefix;suffix) BBa_K587000 RecA Protein coding David Maycotte/ 1062 1103 

                        sequences        Patricia Campos

BBa_K587001 Crxst Regulatory David Maycotte 35 78 BBa_K587002 Tast Regulatory David Maycotte 80 123 BBa_K587003 Tawk Regulatory David Maycotte 79 122 BBa_K587004 Crxwk Regulatory David Maycotte 35 78 BBa_K587005 Itast Regulatory David Maycotte 81 124


*RecA

Sequence

GAATTCGCGGCCGCTTCTAGATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATTGAGAAACAATTTGGTAAAGGCTCCATCATGCGCCTGGGTGAAGACCGTTCCATGGATGTGGAAACCATCTCTACCGGTTCGCTTTCACTGGATATCGCGCTTGGGGCAGGTGGTCTGCCGATGGGCCGTATCGTCGAAATCTACGGACCAGAATCTTCCGGTAAAACCACGCTGACGTTGCAGGTGATCGCCGCAGCGCAGCGCGAAGGTAAAACCTGTGCGTTTATCGATGCTGAACACGCGCTGGACCCAATCTACGCACGTAAACTGGGCGTCGATATTGACAACCTGCTGTGCTCCCAGCCGGATACCGGCGAGCAGGCACTGGAAATCTGTGATGCCCTGGCACGTTCTGGCGCAGTAGACGTTATCGTCGTTGACTCCGTGGCGGCACTGACGCCGAAAGCGGAAATCGAAGGCGAAATCGGCGACTCTCACATGGGCCTTGCGGCACGTATGATGAGCCAGGCGATGCGTAAGCTGGCGGGTAACCTGAAGCAGTCCAACACGCTGCTGATCTTCATCAACCAGATCCGTATGAAAATTGGTGTGATGTTCGGTAACCCGGAAACCACTACCGGTGGTAACGCGCTGAAATTCTACGCCTCTGTTCGTCTCGACATCCGTCGTATCGGCGCGGTGAAAGAGGGCGAAAACGTGGTGGGTAGCGAAACCCGCGTGAAAGTGGTGAAGAACAAAATCGCTGCGCCGTTTAAACAGGCTGAGTTCCAGATCCTCTACGGCGAAGGTATCAACTTCTACGGCGAGCTGGTTGACCTGGGCGTGAAAGAGAAGCTTATCGAGAAAGCAGGCGCGTGGTACAGCTACAAAGGTGAGAAGATCGGTCAGGGTAAAGCGAATGCGACTGCCTGGCTGAAAGATAACCCGGAAACCGCGAAAGAGATCGAGAAGAAAGTACGTGAGTTGCTGCTGAGCAACCCGAACTCAACGCCGGATTTCTCTGTAGATGACAGCGAAGGCGTAGCAGAAACTAACGAAGATTTTTAATACTAGTAGCGGCCGCTGCAG


*Crxst

This DNA sequence acts as a lock for the low arabinose concentration regulation path.

Sequence

GAATTCGCGGCCGCTTCTAGAGAAGCGCAGTAATTCACCTCTTGGATTTGGGTATCATACTAGTAGCGGCCGCTGCAG


*Tast

This sequence acts as the key for the Crxst lock and it unlocks it, in order to activate following mechanism.

Sequence

GAATTCGCGGCCGCTTCTAGAGAGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGAACCTAAAGGGATAGTT TACTAGTAGCGGCCGCTGCAG


*Tawk

This sequence acts as the key fot Crxwk lock and it unlocks it, in order to activate the following mechanism.

Sequence

GAATTCGCGGCCGCTTCTAGAGAGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGATAAACCGTAAGGGTT TACTAGTAGCGGCCGCTGCAG


*Crxwk

This DNA sequence acts as a lock for the high arabinose concentration regulation path.

Sequence

GAATTCGCGGCCGCTTCTAGAGAAGCGCAGTAATTCACCTCTATTTGGCATTCCCAATACTAGTAGCGGCCGCTGCAG


*Itast

This is a complementary sequence for the Tast key, so we call it the antikey since it will inactivate the Tast key and preventing it to interact with the Crxst lock.

Sequence

GAATTCGCGGCCGCTTCTAGAGTCTAGGAGAGATATATGGTATGGTGGTTAATTTTCATTAACCACCAAAGCGCAGTAATTCACCTCTTGGTTTCCCTATCAA TACTAGTAGCGGCCGCTGCAG

Retrieved from "http://2011.igem.org/Team:ITESM_Mexico/Parts_submitted"