Team:Paris Liliane Bettencourt/Notebook/2011/07/17/
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+ | ==Mathias & Laura== | ||
+ | |||
+ | ===Miniprep=== | ||
+ | |||
+ | -RFP & Standard Terminator | ||
+ | |||
+ | -GFP mut3b & T7 Terminator | ||
+ | |||
+ | -pSB1C3 & RBS | ||
+ | |||
+ | ===Digestion and gel extraction=== | ||
+ | |||
+ | -RFP & Standard Terminator by Xba1 & Pst1 | ||
+ | |||
+ | -GFP mut3b & T7 Terminator by Xba1 & Pst1 | ||
+ | |||
+ | -pSB1C3 & RBS by Spe1 & Pst1 | ||
+ | |||
+ | ===Ligation and transformation=== | ||
+ | |||
+ | -RFP Standard Terminator & RBS => K606014 | ||
+ | |||
+ | -GFP mut3b T7 Terminator & RBS => K606013 |
Latest revision as of 16:01, 21 September 2011
Contents |
Mathias & Laura
Miniprep
-RFP & Standard Terminator
-GFP mut3b & T7 Terminator
-pSB1C3 & RBS
Digestion and gel extraction
-RFP & Standard Terminator by Xba1 & Pst1
-GFP mut3b & T7 Terminator by Xba1 & Pst1
-pSB1C3 & RBS by Spe1 & Pst1
Ligation and transformation
-RFP Standard Terminator & RBS => K606014
-GFP mut3b T7 Terminator & RBS => K606013