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Team:Paris Liliane Bettencourt/Notebook/2011/07/10/
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| + | ==Mathias & Laura== | ||
| + | |||
| + | ===Miniprep=== | ||
| + | |||
| + | -pVeg + SpoVg and LacI + double Terminator | ||
| + | |||
| + | -pHyperSpank + SpoVG K143055 | ||
| + | |||
| + | ===Digestion and gel extraction=== | ||
| + | |||
| + | -pVeg + SpoVg and LacI + Term by EcoR1 and Spe1 | ||
| + | |||
| + | -pHyperSpank + SpoVg by EcoR1 and Xba1 | ||
| + | |||
| + | ===Ligation and transformation=== | ||
| + | |||
| + | -pVeg + SpoVg LacI + Term & pHyperSpank + SpoVg => LacI Expression System K606054 | ||
Latest revision as of 15:51, 21 September 2011
Contents |
Mathias & Laura
Miniprep
-pVeg + SpoVg and LacI + double Terminator
-pHyperSpank + SpoVG K143055
Digestion and gel extraction
-pVeg + SpoVg and LacI + Term by EcoR1 and Spe1
-pHyperSpank + SpoVg by EcoR1 and Xba1
Ligation and transformation
-pVeg + SpoVg LacI + Term & pHyperSpank + SpoVg => LacI Expression System K606054
