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Team:Paris Liliane Bettencourt/Notebook/2011/07/07/
From 2011.igem.org
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| + | ==Mathias & Laura== | ||
| + | ===Miniprep=== | ||
| + | |||
| + | -pVeg + SpoVg (K143053) | ||
| + | |||
| + | -LacI + double Terminator | ||
| + | |||
| + | ===Digestion and gel extraction=== | ||
| + | |||
| + | -pVeg + SpoVg by EcoR1 and Spe1 | ||
| + | |||
| + | -LacI + Term (new) by EcoR1 and Xba1 | ||
| + | |||
| + | Transformation following the ligation pVeg + SpoVg and LacI + Term creating the new BioBrick K606010 in pSB1C3 | ||
Latest revision as of 15:42, 21 September 2011
Mathias & Laura
Miniprep
-pVeg + SpoVg (K143053)
-LacI + double Terminator
Digestion and gel extraction
-pVeg + SpoVg by EcoR1 and Spe1
-LacI + Term (new) by EcoR1 and Xba1
Transformation following the ligation pVeg + SpoVg and LacI + Term creating the new BioBrick K606010 in pSB1C3
