Team:ETH Zurich/Process/Validation

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Revision as of 15:27, 21 September 2011

Can you feel the smoke tonight?

Systems Validation

Diffusion-only System

A

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System validation for diffusion

Experimental setup for the diffusion test in agarose filled tubes.

To test if we can create a gradient of a small molecule along our agarose filled tube. We filled a tube (2 mm diameter, 7 cm) long with agarose and E. coli with IPTG-inducible GFP was incubated at 37 °C overnight on end of the tube sticking in 1 ml of 10 mM IPTG solution.

GFP gradient in tube E. coli with IPTG ineducable GFP were incubated in a tube (2 mm diameter, 7 cm long). GFP expression was assessed under the fluorescent microscope after over night with a excitation wavelength of 480 nm and a emission wavelength of 510 nm. The 15 photos were reassembled into one using [http://research.microsoft.com/en-us/um/redmond/groups/ivm/ICE/ the Microsoft Research Image Composite Editor].

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 [[File:Setup_test.png|400px|left|thumb|'''Experimental setup for the diffusion test in agarose filled tubes.''' ]]
-To test if we can create a gradient of a small molecule along our agarose filled tube. We filled a tube (2 mm diameter, 7 cm) long with agarose and E. coli with IPTG inducible GFP was incubated at 37 °C overnight on end of the tube sticking in 1 ml of 10 mM IPTG solution.
+To test if we can create a gradient of a small molecule along our agarose filled tube. We filled a tube (2 mm diameter, 7 cm) long with agarose and ''E. coli'' with IPTG-inducible GFP was incubated at 37 °C overnight on end of the tube sticking in 1 ml of 10 mM IPTG solution.
-[[File:ETHZ Gradient.png|800px|center|thumb|'''GFP gradient in tube''' E.coli with IPTG ineducable GFP were incubated in a tube (2 mm diameter, 7 cm long). GFP expression was assessed under the fluorescent microscope after over night with a excitation wavelength of 480 nm and a emission wavelength of 510 nm.]]
+[[File:ETHZ Gradient.png|800px|center|thumb|'''GFP gradient in tube''' ''E. coli'' with IPTG ineducable GFP were incubated in a tube (2 mm diameter, 7 cm long). GFP expression was assessed under the fluorescent microscope after over night with a excitation wavelength of 480 nm and a emission wavelength of 510 nm. The 15 photos were reassembled into one using [http://research.microsoft.com/en-us/um/redmond/groups/ivm/ICE/ the Microsoft Research Image Composite Editor].]]