Team:ULB-Brussels/mat

From 2011.igem.org

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Materials & Method: page1 </div>
Materials & Method: page1 </div>
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Strain:
 
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E. coli strain:
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<h1>Strain:</h1>
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<h2>E. coli strain:<u></u></h2>
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MC1061 : F− araD139, Δ(ara-leu)7696,galE15, galK16, Δ(lac)X74, rpsL (Strr), hsdR2 (rK−mK+), mcrA mcrB1  
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<ul>
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  <li>MC1061&nbsp;: <em>F−&nbsp;araD139, Δ(ara-leu)7696,galE15, galK16, Δ(lac)X74, rpsL (Strr), hsdR2 (rK−mK+), mcrA mcrB1</em><a href="#_edn1" name="_ednref1" title="" id="_ednref1"> </a></li>
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DG1(Delphi genetics): mcrA Δ (mrr-hsdRMS-mcrBC, modification-,  
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  <li>DG1(Delphi genetics):&nbsp;<em>mcrA&nbsp;Δ (mrr-hsdRMS-mcrBC, modification-, <br />
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restriction-) F80lacZDM15 Δ lacX74 recA1 araD139 Δ (ara-leu)7697 galU galK rpsL endA1 nupG
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    restriction-)&nbsp;F80lacZDM15&nbsp;Δ lacX74 recA1 araD139&nbsp;Δ (ara-leu)7697 galU galK rpsL endA1 nupG</em></li>
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  <li>TOP10 (Invitrogen)&nbsp;: <em>F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-</em></li>
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TOP10 (Invitrogen) : F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-
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  <li>MG1655&nbsp;: <em>F-&nbsp;λ-&nbsp;ilvG- rfb-50 rph-1</em></li>
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  <li>MG1655 <em>ΔtldD::frt-cm-frt</em></li>
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MG1655 : F- λ- ilvG- rfb-50 rph-1
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</ul>
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<h2>Saccharomyces cerevisae strain:</h2>
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MG1655 ΔtldD::frt-cm-frt
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<ul>
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  <li>23344C&nbsp;:<em>ura3</em></li>
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</ul>
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<div>
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Saccharomyces cerevisae strain:
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  <div id="edn1">
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    <p>&nbsp;</p>
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23344C :ura3
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  </div>
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</div>

Revision as of 12:32, 21 September 2011

Materials & Method: page1

Strain:

E. coli strain:

  • MC1061 : F− araD139, Δ(ara-leu)7696,galE15, galK16, Δ(lac)X74, rpsL (Strr), hsdR2 (rK−mK+), mcrA mcrB1
  • DG1(Delphi genetics): mcrA Δ (mrr-hsdRMS-mcrBC, modification-,
    restriction-) F80lacZDM15 Δ lacX74 recA1 araD139 Δ (ara-leu)7697 galU galK rpsL endA1 nupG
  • TOP10 (Invitrogen) : F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-
  • MG1655 : F- λ- ilvG- rfb-50 rph-1
  • MG1655 ΔtldD::frt-cm-frt

Saccharomyces cerevisae strain:

  • 23344C :ura3

 

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